液相芯片技术检测糖尿病患者血清中的多重脂肪因子:应用与优势  

Detection of Multiple Serum Adipokines in Patients with Type 2 Diabetes Mellitus Using Multiplex Analysis Technology: Application and Advantages

作　　者：何欣[1,2] 陆晔[1,2] 朱佩云[1,2] 廖萍[1,2] 刘茶珍[1,2] 王文静[1,2] 

机构地区：[1]上海市疾病预防控制中心慢性非传染病与伤害防治所,上海200336 [2]上海市预防医学研究院,上海200336

出　　处：《环境与职业医学》2016年第6期557-561,共5页Journal of Environmental and Occupational Medicine

基　　金：上海市卫生和计划生育委员会面上项目(编号:201440617)

摘　　要：[目的]利用液相芯片技术检测多重脂肪因子在老年糖尿病患者和对照人群血清中的水平差异。[方法]采用Magpix液相芯片仪对28名老年糖尿病患者和22名正常对照者血清中的脂联素、抵抗素、脂肪酶、脂质运载蛋白-2及纤溶酶原激活物抑制剂-1的表达水平进行检测,并分析两组人群各种脂肪因子水平差异。[结果]在糖尿病患者血清中纤溶酶原激活物抑制剂-1浓度是对照组的1.29倍(P<0.05),其他4种脂肪因子水平在两组间差异无统计学意义(P>0.05)。[结论]血清纤溶酶原激活物抑制剂-1浓度与糖代谢密切相关,液相芯片技术的应用为慢性病的生物监测和控制提供了极大的便利条件。[Objective] To detect the levels of multiple serum adipokines in elderly patients with type 2 diabetes and healthy controls by multiplex analysis technology. [Methods] A total of 28 cases of elderly patients with type 2 diabetes mellitus（T2DM） and 22 cases of healthy controls were recruited in this study. Levels of serum multiple adipokines including adiponectin, resistin, adipsin, lipocalin-2, and plasminogen activator inhibitor-1（PAI-1） were detected by the Magpix liquid chip instrument. The difference of the levels between the two groups was analyzed. [Results] Only serum PAI-1 level of the T2 DM group was 1.29 times that of the control group（P〈0.05）, whereas other four adipokines had no differences（P〉0.05）. [Conclusion] PAI-1 level is closely associated with glycometabolism. Multiplex analysis technology facilitates chronic disease biomonitoring and control.

关 键 词：液相芯片技术 Magpix液相芯片仪 2型糖尿病(T2MD) 脂肪因子 

分 类 号：R122.12[医药卫生—环境卫生学]