苯暴露对小鼠血液系统和DNA氧化损伤的影响  

Effects of Benzene on Blood System and DNA Oxidative Damage in Mice

作　　者：丁婷婷[1] 王茜[1] 郑国颖[1] 刘英莉[1] 袁聚祥[1] 沈福海[1] 蒋守芳[1] 冯福民[1] 金玉兰[1] 关维俊[1] 佟俊旺[1] 

机构地区：[1]华北理工大学公共卫生学院劳动卫生与环境卫生学科、河北省煤矿卫生与安全实验室,河北唐山063000

出　　处：《环境与职业医学》2016年第6期571-574,共4页Journal of Environmental and Occupational Medicine

基　　金：河北省科技计划项目(编号:13277709D)

摘　　要：[目的]观察苯吸入染毒对小鼠的周围血象、尿中苯巯基尿酸(SPMA)和8-羟基脱氧鸟苷(8-OHd G)的影响,探讨苯暴露对血液系统和DNA氧化损伤的作用。[方法]将120只昆明种雄性小鼠随机分为4组,每组30只,低、中、高组苯染毒质量浓度分别为375、750和1 500 mg/m3,对照组吸入空气。采用静式吸入染毒法,每天2 h,每周5 d,持续染毒3个月。分别于染毒后1、2、3个月时,每组随机处死10只小鼠,检测周围血象、尿中SPMA和8-OHd G的水平。[结果]白细胞、红细胞、淋巴细胞计数均随着染毒浓度的升高而降低(P<0.05);尿中SPMA及8-OHd G水平随着苯染毒浓度的增加而升高(P<0.05)。高浓度组染毒3个月时尿中8-OHd G水平明显高于1、2个月(P<0.05)。尿中SPMA及8-OHd G与白细胞计数均呈负相关(rSPMA=-0.718,r8-OHd G=-0.971,均P<0.01)。[结论]亚慢性苯暴露可导致小鼠血液系统和DNA氧化损伤。[Objective] To observe the changes of peripheral hemogram, urinary S-phenylmercapturic acid（SPMA）, and 8-hydroxy-2＇-deoxyguanosine（8-OHd G） in mice as indicators of blood system and DNA oxidative damage after benzene inhalation. [Methods] A total of 120 Kunming male mice were exposed to 0, 375, 750, and 1 500 mg/m3 of benzene for 2 h each day, 5 days each week, in static inhalation cabin for three months. In each group ten mice were randomly sacrificed every month. Hemogram and urinary SPMA and 8-OHd G were measured. [Results] With higher benzene exposure dose, the counts of white blood cells（WBC）, red blood cells（RBC）, and lymphocytes were reduced（P〈0.05）, but the levels of SPMA and 8-OHd G in urine were increased（P〈0.05）. Higher urinary 8-OHd G level was found in the mice with three months of 1500 mg/m3 benzene inhalation than those with one or two months of exposure（P〈0.05）. Urinary SPMA and 8-OHd G were negatively correlated with WBC（rSPMA=-0.718, r8-OHd G=-0.971, P〈0.01）. [Conclusion] Subchronic exposure to benzene might induce blood system and DNA oxidative damage in mice.

关 键 词：苯 静式吸入染毒 血象 苯巯基尿酸 8-羟基脱氧鸟苷 

分 类 号：R135.12[医药卫生—劳动卫生]