右旋美托咪啶对福尔马林炎性痛大鼠吗啡耐受的调节及机制  

Reverse regulation and mechanism of dexmedetomidine on formalin inflammatory pain and morphine tolerance in rats

作　　者：何莉[1] 郭静雯[1] 

机构地区：[1]广州市第一人民医院麻醉科,510180

出　　处：《白求恩医学杂志》2016年第3期275-277,共3页Journal of Bethune Medical Science

基　　金：广州市医药卫生科技项目(编号:20151A010001)

摘　　要：目的观察右旋美托咪啶（Dex）对福尔马林炎性痛大鼠吗啡耐受的调节及机制。方法选取雄性SD大鼠40只,采用5%福尔马林建立急性炎性疼痛模型,随机分为4组,各10只。Ⅰ组（空白组）、Ⅱ组（吗啡耐受组）、Ⅲ组（Dex组）、Ⅳ组（Dex＋NMDA组）。观察脊髓背角神经元型一氧化氮合酶（n NOS）、信使核糖核酸（mRNA）的表达及一氧化氮（NO）含量与一氧化氮合酶（NOS）活性。结果脊髓背角n NOS及mRNA在Ⅰ~Ⅳ组均有表达,组间脊髓背角n NOS及mRNA比较,差异具有统计学意义（P〈0.01）;其中Ⅲ组n NOS及mRNA相对表达量与Ⅰ组无明显差异（P〉0.05）,但均低于Ⅱ、Ⅳ组（P〈0.05）。组间脊髓背角NO含量及NOS活性比较,差异具有统计学意义（P〈0.01）;其中Ⅲ组大鼠的脊髓背角NO含量和NOS活性与Ⅰ组比较无明显差异（P〉0.05）,但均低于Ⅱ、Ⅳ组（P〈0.05）。结论在福尔马林炎性痛大鼠模型中,Dex可抑制脊髓背角n NOS、mRNA表达及NO产生,使吗啡耐受得到逆转。Objective To observe the effects of Dex on formalin inflammatory pain and morphine tolerance in rats and its mechanism. Methods Selected forty male SD rats,using 5% formalin to establish acute inflammatory pain model,were randomly divided into 4 groups,10 rats in each group. Group I（ blank group）,morphine tolerance group（ morphine tolerance group）,group III（ group Dex）,group IV（ group Dex ＋ NMDA）. Expression of neuronal nitric oxide synthase（ n NOS）,messenger RNA（ mRNA） and nitric oxide（ NO） content and nitric oxide synthase（ NOS） activity in spinal dorsal horn neurons were observed. Results The expression of nNOS in spinal dorsal horn and mRNA in group I,group II,group III,IV group were,the group III of n NOS mRNA and the relative expression quantity and group I had no significant difference（P〈0. 05） but lower than that of group II and group IV（P〈0. 05）; group III rats spinal cord dorsal horn of no content and NOS activity and group I no statistical significance（P〈0. 05）,but compared with group II and IV group,no content were reduced 74. 5% and 40%,NOS activity were decreased by 55. 0% and 31. 4%（P〈0. 05）. Conclusion Among rats of the formalin inflammatory pain model,DEX can inhibit n NOS in spinal dorsal horn,mRNA expression and NO production,so morphine tolerance has been reversed.

关 键 词：右旋美托咪啶 天冬门氨酸 神经元型一氧化氮合酶 福尔马林 吗啡 

分 类 号：R96[医药卫生—药理学]