大黄素对人胃癌细胞株SGC-7901凋亡的影响与初步机制  

Effect of emodin on apoptosis in human stomach cancer cell line SGC-7901 and possible mechanisms involved

作　　者：马陈皋 

机构地区：[1]浙江中医药大学第二临床医学院,浙江省杭州市310053

出　　处：《世界华人消化杂志》2016年第16期2463-2469,共7页World Chinese Journal of Digestology

摘　　要：目的:探讨大黄素诱导人胃癌细胞株SGC-7901凋亡及其相关机制.方法:采用CCK-8法和Tunel染色法检测不同浓度大黄素处理后,SGC-7901细胞凋亡的变化;免疫印迹法检测Bcl-2、Bax、cleavedCaspase3、cleaved-PARP、pre Caspase3、PARP的蛋白表达水平;JC-1染色荧光显微观察线粒体膜电位的变化.结果:CCK-8法和Tunel染色法显示大黄素呈浓度依赖性促进SGC-7901细胞的凋亡(存活率100.73%±8.97%vs 45.27%±3.75%,P<0.05);免疫印迹法显示给予60μm o l/L大黄素处理后,与空白对照组相比,细胞中B c l-2的蛋白表达显著降低(0.31±0.02 v s1.01±0.06,P<0.05),而B a x的表达显著增加(1.98±0.12 vs 1.00±0.08,P<0.05),导致Bcl-2/Bax比值显著降低(0.14±0.01 vs 1.02±0.13,P<0.05);再者,JC-1染色显示线粒体膜电位降低;活性的cleaved-Caspase3(1.73±0.13 vs 0.98±0.06,P<0.05)、cleavedPARP(2.29±0.17 vs 1.01±0.08,P<0.05)表达增加.结论:大黄素呈浓度依赖性促进SGC-7901细胞的凋亡,其作用机制可能与线粒体途径凋亡有关.AIM： To investigate the effect of emodin on cell apoptosis in human stomach cancer cell line SGC-7901 and to explore the related mechanisms.METHODS： After SGC-7901 cells were treated with different concentrations of Emodin, cell apoptosis was determined by CCK-8 assay and TUNEL assay. The expression of Bcl-2, Bax, cleaved-Caspase3, cleaved-PARP, preC aspase3, and PARP was measured by Western blot. Mitochondrial membrane potential was examined by JC-1 staining and fluorescence microscopy. RESULTS： The apoptotic rate of SGC-7901 cells was increased by emodin in a dosedependent manner（survival rate 100.73% ± 8. 9 7 % v s 4 5. 2 7 % ± 3. 7 5 %, P〈0. 0 5）. Compared with the control group, treatment with emodin（ 6 0 μ mol / L） significantly increased Bax expression（1.98 ± 0.12 vs 1.00 ± 0.08, P〈0.05）, Caspase3（1.73 ± 0.13 vs 0.98 ± 0.06, P〈0.05） and PARP（2.29 ± 0.17 vs 1.01 ± 0.08, P〈0.05） activation, while decreased Bcl-2 expression（0.31 ± 0.02 vs 1.01 ± 0.06, P〈0.05）, Bcl-2/Bax ratio（0.14 ± 0.01 vs 1.02 ± 0.13, P〈0.05） and mitochondrial membrane potential. CONCLUSION： Emodin accelerates the apoptosis o f SGC-7901 cells via the mitochondria-dependent pathway. Our results reveal a novel role for emodin in the treatment of gastric cancer.

关 键 词：大黄素 人胃癌细胞株SGC-7901 凋亡 线粒体 

分 类 号：R285[医药卫生—中药学]