慢性乙型肝炎患者的差异表达基因筛选及相关信号通路预测  

Screening of differentially expressed genes in chronic hepatitis B patients and prediction of related biological pathways

作　　者：宁文锋[1] 王芳[1] 邓宏军[1] 陈宏辉[1] 

机构地区：[1]南华大学附属第二医院消化内科,湖南省衡阳市421001

出　　处：《世界华人消化杂志》2016年第16期2485-2491,共7页World Chinese Journal of Digestology

基　　金：衡阳市科技局基金资助项目;No.2010ks16;湖南省卫生厅基金资助项目;No.C2013-013~~

摘　　要：目的:研究慢性乙型肝炎发病的分子机制.方法:通过基因表达谱芯片,运用Gene Spring软件筛选慢性乙型肝炎患者的差异表达基因;运用Gene Trail软件,对差异表达基因进行信号通路富集分析.结果:发现417个差异表达基因,其中表达上调的基因数目为205个,表达下调的基因数目为212个;下调基因的显著性信号通路为ErbB、非小细胞肺癌、mTOR、RNA降解、T细胞受体、慢性粒细胞白血病、肾细胞癌信号通路,上调基因的显著性信号通路为趋化因子、溶酶体、霍乱弧菌感染、IgGFc受体介导的吞噬作用信号通路.结论:PI3K/AKT下调可能是乙型肝炎患者持续感染的主要分子机制之一.AIM： To study the molecular mechanism of pathogenesis of chronic hepatitis B. METHODS： Based on microarray experiment, Gene Spring software was used to screen differentially expressed genes in chronic hepatitis B patients, and GeneT rail software was used to perform enrichment analysis of related biological pathways. RESULTS： A total of 417 differentially expressed genes were identified, of which 205 were upregulated and 212 downregulated. Significant pathways to which downregulated genes belong include ErbB, non-small cell lung cancer, mTOR, RNA degradation, T cell receptor, chronic myeloid leukemia, and renal cell carcinoma pathways. Significant pathways to which upregulated genes belong include chemokine, lysosomes, Vibrio cholerae infection, and IgGFc receptor-mediated phagocytosis pathways. CONCLUSION： PI3K/AKT downregulation is likely a major molecular mechanism of persistent hepatitis B.

关 键 词：慢性乙型肝炎 基因芯片 差异表达基因 信号通路 

分 类 号：R512.62[医药卫生—内科学]