茵陈蒿汤对梗阻性黄疸大鼠肝细胞中IRE1α蛋白表达的影响  

Effect of Yinchenhao decoction on expression of IRE1α protein in liver cells of rats with obstructive jaundice

作　　者：宋仕军[1] 李忠廉[2] 张西波[2] 

机构地区：[1]天津医科大学研究生院,天津市300070 [2]天津市南开医院肝胆胰外科,天津市300100

出　　处：《世界华人消化杂志》2016年第16期2520-2524,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81273952~~

摘　　要：目的:研究茵陈蒿汤血清对梗阻性黄疸大鼠肝细胞中肌醇酶1α(inositol-requiring enzyme-1-α,IRE1α)蛋白表达的影响.方法:通过结扎SD大鼠胆总管建立梗阻性黄疸模型,分离梗阻性黄疸大鼠原代肝细胞进行体外培养.通过喂食SD大鼠茵陈蒿汤制备相应的含药血清.处理组(B组)以含药血清培养基培养大鼠原代肝细胞,对照组(A组)以不含药的常规培养基培养大鼠原代肝细胞.分别测定细胞中IRE1α蛋白表达情况及培养液中谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)含量.结果:通过比较对照组(A组)各时间点与处理组(B组)的蛋白和转氨酶含量,B组在I R E1α蛋白表达(I R E1α表达量:6 h 1.85±0.04 vs 1.58±0.04,24 h 1.95±0.02 vs 1.60±0.03,48 h 2.22±0.13 vs 1.99±0.10,P<0.05)及ALT、AST测定值均降低(AST测定值:6 h17.23 U/L±3.01 U/L vs 13.13 U/L±2.41 U/L,24 h 19.33 U/L±3.01 U/L vs 15.67 U/L±2.36U/L,48 h 24.40 U/L±3.93 U/L vs 19.18 U/L±1.65 U/L,P<0.05;ALT测定值:6 h 17.23U/L±3.01 U/L vs 13.13 U/L±2.41 U/L,24 h19.33 U/L±3.01 U/L vs 15.67 U/L±2.36 U/L,48 h 24.40 U/L±3.93 U/L vs 19.18 U/L±1.65U/L,P<0.05),差异有统计学意义.结论:血清药理学研究方法可以用于茵陈蒿汤的药效评价,经IRE1α介导的内质网应激(endoplasmic reticulum stress,ERS)可能是茵陈蒿汤对梗阻性黄疸大鼠肝细胞保护作用的一条重要通路.AIM： To investigate the effect of medicated serum of Yinchenhao decoction on inositolrequiring enzyme-1-α（IRE1α） protein expression in hepatocytes of rats with obstructive jaundice.METHODS： Common bile duct ligation was used to generate an SD rat model of obstructive jaundice. Primary hepatocytes were isolated from the rat models. Gavage administration with Yinchenhao decoction was applied to prepare serum containing the corresponding drug. Yinchenhao decoction serum was added into the medium for primary hepatocyte culture in the administrated group（Group B）, while the medium containing no drug was used in the sham group（Group A）. The expression of IRE1α protein in cells as well as alanine transaminase（ALT） and aspartate transaminase（AST） in culture fluid was detected.RESULTS： The levels of IRE1α expression, AST and ALT in group B were significantly lower than those in group A at all time points（expression of IRE1α： 6 h 1.85 ± 0.04 vs 1.58 ± 0.04, 24 h 1.95 ± 0.02 vs 1.60 ± 0.03, 48 h 2.22 ± 0.13 vs 1.99 ± 0.10, P〈0.05; AST： 6 h 17.23 U/L ± 3.01 U/L vs 13.13 U/L ± 2.41 U/L, 24 h 19.33 U/L ± 3.01 U/L vs 15.67 U/L ± 2.36 U/L, 48 h 24.40 U/L ± 3.93 U/L vs 19.18 U/L ± 1.65 U/L, P〈0.05; ALT： 6 h 17.23 U/L ± 3.01 U/Lvs 13.13 U/L ± 2.41 U/L, 24 h 19.33 U/L ± 3.01U/L vs 15.67 U/L ± 2.36 U/L, 48 h 24.40 U/L ±3.93 U/L vs 19.18 U/L ± 1.65 U/L, P〈0.05）.C ONCLUSION ： Serum pharmacology test can be used for pharmacodynamic evaluation of Yinchenhao decoction. The endoplasmic reticulum stress mediated by IRE1α might be an important pathway by which Yinchenhao decoction exerts a protective effect on hepatocytes of rats with obstructive jaundice.

关 键 词：茵陈蒿汤 血清药理学 肌醇酶1 内质网应激 梗阻性黄疸 

分 类 号：R285.5[医药卫生—中药学]