高效液相色谱法测定豆芽中痕量2,4-二氯苯氧乙酸  

Determination of the trace amounts of 2,4-D in bean sprout samples by HPLC with 2,4-Dichlorophenoxyacetic acid

作　　者：田莉莉[1] 刘晓玲[1] 刘霞[1] 

机构地区：[1]银川能源学院石化系,银川750105

出　　处：《粮食与食品工业》2016年第3期72-75,共4页Cereal & Food Industry

基　　金：银川能源学院科技开发与应用研究基金项目

摘　　要：以氯甲基化交联聚苯乙烯树脂（CMCPS）为载体和大分子引发剂,2,4-二氯苯氧乙酸（2,4-D）为模板,丙烯酰胺（AM）为单体,采用表面引发原子转移自由基聚合（SI-ATRP）技术,制备了2,4-二氯苯氧乙酸分子印迹聚合物（MIPs）,并将该聚合物作为固相萃取剂,以高效液相色谱法测定豆芽样品中的痕量2,4-D,加标回收率为86%~104%,相对标准偏差为1.9%~10%,检出限为20 ng/g。该印迹材料稳定性好,且有良好的再生性能。2,4-dichlorophenoxyacetic acid imprinted materials（2,4-D MIPs）were prepared by surface-initiated atom transfer radical polymerization（SI-ATRP）method.The chloromethyl polystyrene resin（CMCPS）as carrier and macroinitiator for the first time,the imprinted polymers were carried out by using 2,4-dichlorophenoxyacetic acid as template,acrylic amide as functional monomer and ethylene glycol dimethacrylate（EDMA）as cross-linker,CuBr/2,2＇-bipyridine（Bpy）as the catalytic system.2,4-D MIPs as solid phase extractant,the addition standard recoveries are 86% ~104% with RSD of 1.9% ~10%,the limit of detection（LOD）in the bean sprout samples is 20 ng/g.The imprinted material is stable and can be used repeatedly.

关 键 词：表面引发原子转移自由基聚合 分子印迹聚合物 2 4-二氯苯氧乙酸 固相萃取 豆芽样品 HPLC 

分 类 号：TS27[轻工技术与工程—农产品加工及贮藏工程]