黄精多糖对糖尿病大鼠心肌纤维化影响  

Effect of Polygonatum sibiricum polysaccharides on myocardial fibrosis in rats with diabetes

作　　者：张忠英[1] 王国贤[1] 陈婷婷[1] 付婷婷[1] 

机构地区：[1]辽宁医学院药理教研室,辽宁锦州121001

出　　处：《中国公共卫生》2016年第6期807-810,共4页Chinese Journal of Public Health

摘　　要：目的观察黄精多糖(PSP)对糖尿病心肌病大鼠心肌纤维化影响,探讨其可能机制。方法健康雄性SD大鼠,随机分为对照组、模型组、黄精多糖250、500、1000 mg/kg组,所有大鼠给予普通饲料,连续12周,观察各组大鼠一般情况,检测空腹血糖,血生化指标,血流动力学指标,计算心脏质量指数(HMI),左心室质量指数(LVMI),苏木素-伊红染色观察心肌形态学变化;Masson染色观察心肌胶原纤维分布;免疫组化法检测心肌组织骨形态发生蛋白-7(BMP-7)、转化生长因子β1(TGF-β1)、Smad2/3、Smad7蛋白表达。结果与对照组比较,模型组大鼠空腹血糖[(24.81±2.30)mmol/L]、HMI[(4.16±1.14)]、LVMI[(2.85±0.09)]及LVEDP升高(P<0.05);免疫组化结果显示,模型组大鼠心肌组织中TGF-β1、Smad2/3蛋白的表达显著增加(P<0.05),BMP-7、Smad7蛋白表达明显减少(P<0.05);HE、M asson染色显示模型组大鼠心肌组织病理损伤严重。与模型组比较,黄精多糖1 000 mg/kg组大鼠空腹血糖[(13.28±2.08)mmol/L]、HMI[(3.21±0.07)]、LVMI[(2.09±0.02)]及LVEDP下降(P<0.05),心肌组织中TGF-β1、Smad2/3蛋白表达显著减少(P<0.05),BM P-7、Smad7蛋白表达明显升高(P<0.05);心肌组织病理改变有所改善。结论黄精多糖可改善糖尿病大鼠心肌纤维化,其机制可能与促进心肌组织中BMP-7表达,影响TGF-β1/Smads信号通路有关。Objective To observe the effect of Polygonatum sibiricum polysaccharides （PSP） on myocardial fibro- sis in diabetic cardiomyopathy rats and to explore its possible mechanism. Methods Healthy male Sprague-Dawkey rats were randomly divided into normal group,model group,and 250,500,1000 mg/kg PSP groups and all the rats were fed with normal diet for 12 consecutive weeks. General conditions of the rats in each group were observed;fasting blood glu- cose （FBG） ,blood biochemical indexes ,hemodynamic indexes were detected and heart mass index （HMI） ,left ventricu- lar mass index （LVMI）, and left ventricular end-diastolic pressure （LVEDP）were determined. Hematoxylin-eosin staining was used to observe the changes of myocardial morphology;Masson staining was used to observe the distribution of colla- gen fiber;the expressions of the bone morphogenetic protein-7 （BMP-7）,transforming growth factor beta I （TGF-131）, Smad2/3 and Smad7 were detected with immunohistochemistry. Results Compared with those of the control group, the FBG （24. 81 ±2. 30 mmol/L） ,HMI （4. 16 ± 1.14） ,LVMI （2. 85 ±0. 09） and LVEDP of the rats in the model group were increased （ all P 〈 0. 05 ）. Immunohistochemistry results showed that the expressions of TGF-β1 and Smad2/3 protein were significantly increased （ P 〈 0. 05 ） ; the expressions of BMP-7 and Smad7 protein were significantly reduced （ P 〈 0. 05 ） ; HE and Masson staining showed that the cardiac tissues were serious damaged for the rats in the model group. Compared with those of the model group, the FBG （13.28 ± 2.08 mmol/L）, HMI （3.21± 0. 07 ）, LVMI （2. 09± 0. 02 ）, and LVEDP were decreased （ all P 〈 0. 05 ） ; the expressions of TGF-β1 and Smad2/3 protein were significantly reduced （ both P 〈 0. 05 ） ; the expressions of BMP-7 and Smad7 protein were significantly increased （ both P 〈 0.05 ） ; and the pathological changes of cardiac tissue were improved for the rats in 1000 mg/kg PSP treatment group

关 键 词：黄精多糖(PSP) 心肌纤维化 骨形态发生蛋白-7(BMP-7) 转化生长因子β1(TGF-β1)/Smads SMAD7 

分 类 号：R587.1[医药卫生—内分泌]