八角茴香不同加工品反式茴香脑含量及温阳散寒、止痛作用的药效作用比较  

Comparison of Illicium Verum Pharmacodynamic Effect of Different Processing Products of Trans-anethole Content as Well as Warming Yang for Dispelling Cold and Analgesic Effect

作　　者：谢滟[1] 邓家刚[1] 黄丽贞[1] 何育佩 吴秋玲[1] 韦林垚 李云娟[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《科学技术与工程》2016年第16期149-154,共6页Science Technology and Engineering

基　　金：国家科技支撑计划(2011BAI01B04);广西中药药效研究重点实验室建设(13-051-06)资助

摘　　要：采用气体色谱(GC)法测定八角茴香不同加工品的八角茴香反式茴香脑含量;并以药效评价八角茴香不同加工品的温阳散寒及止痛作用。采用无水乙醇分别提取微波火力60%烘干、光波烘干、晒干、阴干方法加工的八角茴香的反式茴香脑,GC法测定其含量。采用龙胆草、黄柏、生石膏和知母四味寒凉药水煎液灌胃复制实寒证大鼠模型,观察八角茴香不同加工品对实寒证大鼠能量代谢的影响;采用4℃冰醋酸造成小鼠腹部冷痛,观察八角茴香止痛作用。八角茴香微波火力60%烘干品、光波烘干品、晒干品、阴干品反式茴香脑平均含量分别为104.51、102.25、105.82、103.08,反式茴香脑含量无明显区别;微波八角组可升高血浆及肝匀浆中丙酮酸含量;光波八角组可升高血浆中丙酮酸、甘油三酯含量;八角八角、光波八角组、晒干八角组可升高肝匀浆中Ca^(2+)-Mg^(2+)-ATP酶活力;微波八角、晒干八角及阴干八角组可降低肝匀浆中乳酸含量;4种加工品均可减少4℃冰醋酸引起的小鼠扭体反应次数。八角茴香4种加工品反式茴香脑含量无明显区别,均具有温阳散寒、止痛作用。GC was adopted to determine the illicium verum of different processing products of trans-anethole content,and through the efficacy evaluation of illicium verum in different processed products of warming yang for dispelling cold as well as analgesic effect. Using the absolute alcohol separately the trans-anethole from the 60% of microwave power,the light power,the sun drying,the shadow drying,and determine the content were extracted. The rats decoction was given that can lead them to appear the symptoms of excess-cold,the water of cold traditional chinese medicine were conduct with gentian,phellodendron,gypsum and rhizome anemarrhenae,giving the water to rats by gastric gavage,observe the effect of energy metabolism in rats by given the illicium verum in different processed products; giving the glacial acetic acid of 4 ℃ which can caused the mouse abdominal pain,and observe the effect of analgesia. Four illicium verum products,（ the 60% of microwave power,the light power,the sun drying,the shadow drying） average content of trans-anethole were 104. 51,102. 25,105. 82 and 103. 08 respectively,no obvious differences of the content,the microwave group can rise the content of PA in the blood plasma and the liver tissue homogenate,the light group can rise the content of PA,TG in the blood plasma,the groups of microwave、light and the sun drying all can raise the vitality of LDH,Ca^（2＋）-Mg^（2＋）-ATPase in the liver tissue homogenate,meanwhile the group of microwave,the sun drying and the shadow drying can reduce the content of LD in the liver tissue homogenate,at the same time. Four illicium verum products all can reduce the mice writhing reaction times after giving the 4 ℃ glacial acetic acid. There were no obvious differences of the trans-anethole content from four illicium verum products,they all have the function of warming yang for dispelling cold and analgesia.

关 键 词：八角茴香 微波火力60%品 光波品 晒干品 阴干品 温阳散寒 止痛 

分 类 号：R285.5[医药卫生—中药学]