辛伐他汀对慢性阻塞性肺疾病大鼠肺部及系统性炎症反应的影响  

Influence of simvastatin on pulmonary and systemic informatory responses in COPD rats

作　　者：黄炎明[1] 熊萍[1] 左万里[1] 张春来[1] 周伟[2] 

机构地区：[1]江门市中心医院呼吸内科,广东江门529030 [2]江门市中心医院病理科,广东江门529030

出　　处：《中国临床研究》2016年第6期730-733,共4页Chinese Journal of Clinical Research

摘　　要：目的探讨辛伐他汀早期干预对慢性阻塞性肺疾病(慢阻肺)大鼠肺部及系统性炎症反应的影响。方法将40只雄性Wistar大鼠随机均分为正常组(A组)、辛伐他汀组(B组)、慢阻肺模型组(C组)、辛伐他汀干预组(D组),每组10只。C组和D组采用反复香烟烟雾暴露法制备慢阻肺模型。B组和D组大鼠采用辛伐他汀5 mg·kg^(-1)·d^(-1)灌胃,其他两组用等体积的生理盐水灌胃,共16周。造模结束后留取血液标本测定系统性炎症指标[外周血白细胞计数(WBC)及血清超敏C反应蛋白(hs-CRP)];制作肺组织病理切片进行病理学观察,计数平均肺内衬间隔(MLI)和平均肺泡数(MAN)。结果 C组与D组的气道炎症病理评分、MLI、外周血WBC及血清hs-CRP水平均较A、B组明显增高(P均<0.01),MAN则较A、B组明显减低(P均<0.01)。与C组比较,D组气道炎症病理评分、MLI、外周血WBC及血清hs-CRP均明显减低(P均<0.01),MAN较C组增高(P<0.01)。外周血WBC、血清hs-CRP水平与气道内炎症病理评分成正相关(r=0.598,P<0.01;r=0.767,P<0.01),与MAN则成负相关(r=-0.657,P<0.01;r=-0.702,P<0.01)。结论辛伐他汀可抑制肺部及系统性炎症反应,减轻肺气肿的程度,从而有可能延缓肺功能的下降。Objective To explore the influence of simvastatin early intervention on the pulmonary and systemic informatory responses in rats with chronic obstructive pulmonary disease（ COPD）. Methods Forty male Wistar rats were randomly divided into four groups（ n = 10 each） ： control group（ group A）,simvastatin group（ group B）,COPD group（ group C）and simvastatin intervention group（ group D）. The COPD model was established by repeatedly exposing rats to the smog environment of cigarette in group C and D. Simvastatin（ 5 mg·kg^（-1）·d^（-1）） was given by gavage in group B and D,and equal volume of normal saline gavage was given in group A and C for 16 weeks. After modeling,the blood samples were taken to detect systemic inflammatory indexes including white cell count of peripheral blood（ WBC） and serum hypersensitive Creactive protein（ hs-CRP）. Pathological section of lung tissue was made to observe the pathology and calculate pulmonary mean linear intercept（ MLI） and mean alveolar number（ MAN）. Results Compared with group A and B,the airway inflammation and pathology scores,MLI,WBC of peripheral blood and serum hs-CRP levels significantly increased,while MAN significantly decreased in group C and D（ all P 0. 01）. Compared with group C,the airway inflammation and pathology scores,MLI,WBC of peripheral blood and serum hs-CRP levels significantly decreased,while MAN significantly increased in group D（ all P 0. 01）. WBC of peripheral blood and serum hs-CRP levels were positively correlated with airway inflammation and pathology scores（ r = 0. 598,P 0. 01; r = 0. 767,P 0. 01）,while were negatively correlated with MAN（ r =- 0. 657,P 0. 01; r =- 0. 702,P 0. 01）. Conclusions Simvastatin can inhibit the pulmonary and systemic informatory responses,reduce the degree of emphysema,thus it may delay the decline of lung functions.

关 键 词：慢性阻塞性肺疾病 辛伐他汀 系统性炎症反应 气道炎症病理学评分 肺内衬间隔 肺泡数 

分 类 号：R33[医药卫生—人体生理学] R563[医药卫生—基础医学]