急性自发性气胸患者肺大疱中细胞因子的表达及其意义  

Expressions and significance of cytokines in pulmonary bullae in patients with acute spontaneous pneumothorax

作　　者：杨罡 王争君[2] 朱琳燕[2] 梁毅[2] 

机构地区：[1]鄂东医疗集团黄石市中医医院内一科,湖北黄石435000 [2]鄂东医疗集团黄石市中心医院心胸外科,湖北黄石435000

出　　处：《中国临床研究》2016年第6期737-740,共4页Chinese Journal of Clinical Research

基　　金：2013年湖北省自然科学基金项目(2013CFC060)

摘　　要：目的探讨成纤维生长细胞因子-10(FGF-10)、肺泡细胞表面抗原-6(KL-6)、基质金属蛋白酶-9(MMP-9)及人组织型基质金属蛋白酶抑制剂-1(TIMP-1)在急性自发性气胸患者肺大疱组织中的表达及其意义。方法选取黄石市中心医院2013年1月至2015年9月手术治疗的47例自发性气胸患者作为研究对象,采用免疫组织化学染色法观察肺大疱及疱旁正常组织标本中FGF-10、KL-6、MMP-9及TIMP-1蛋白的表达水平。结果肺大疱组织中FGF-10、KL-6蛋白占总蛋白的比值分别为0.0565±0.0108、0.1482±0.0731,均显著高于疱旁组织的0.0118±0.0062、0.0561±0.0203,差异均具有统计学意义(P均<0.01);肺大疱组织中MMP-9及TIMP-1蛋白占总蛋白的比值分别为0.0037±0.0015、0.0069±0.0017,与疱旁组织中占总蛋白的比值0.0033±0.0013、0.0063±0.0020比较,差异均无统计学意义(P均>0.05)。肺大疱组织中FGF-10、KL-6蛋白着色程度较疱旁组织显著加深,而MMP-9及TIMP-1蛋白着色程度与疱旁组织差别不明显。结论 KL-6介导的肺纤维化及FGF-10在肺组织中过表达可能与肺大疱形成有关,MMP-9及TIMP-1在肺大疱中的表达变化不明显。Objective To investigate the expressions of fibroblast growth factor-10（ FGF-10）,alveolar cell surface antigen6（ KL-6）,matrix metalloproteinase-9（ MMP-9） and human tissue inhibitors of metalloproteinase-1（ TIMP-1） in the tissues of pulmonary bullae in patients with acute spontaneous pneumothorax and their significances. Methods The samples of pulmonary bullae tissues and bullae-adjacent normal tissues from 47 patients with spontaneous pneumothorax treated surgically in Huangshi Central Hospital from January 2013 to September 2015 were selected as research objects. Immunohistochemical method was used to detect the contents of FGF-10,KL-6,MMP 9 and TIMP-1 proteins for calculating the ratios of cytokins to total proteins quantity of samples and their expressions in tissues. Results The ratios of FGF-10 and KL-61 proteins quantities to total proteins quantity of samples（ 0. 0565 ± 0. 0108,0. 1482 ± 0. 0731） in pulmonary bullae tissues were significantly higher than those in bullae-adjacent normal tissues（ 0. 0118 ± 0. 0062,0. 0561 ± 0. 0203）（ all P 0. 05）. The ratios of MMP-9 and TIMP-1 proteins quantities to total proteins quantity of samples（ 0. 0037 ± 0. 0015,0. 0069 ± 0. 0017） in pulmonary bullae tissues were similar with bullae-adjacent normal tissues（ 0. 0033 ± 0. 0013,0. 0063 ±0. 0020）（ all P 0. 05）. The coloring degrees of FGF-10 and KL-61 significantly deepened in pulmonary bullae tissues compared with bullae-adjacent normal tissues,but the coloring degrees of MMP-9 and TIMP-1 in pulmonary bullae tissues were similar with bullae-adjacent normal tissues. Conclusions The KL-6-mediated pulmonary fibrosis and FGF-10 overexpression in pulmonary tissues may be associated with lung bullae formation. MMP-9 and TIMP-1 expressions in pulmonary bullae tissues have no obvious changes compared with normal pulmonary tissues.

关 键 词：成纤维生长细胞因子-10 肺泡细胞表面抗原-6 基质金属蛋白酶-9 基质金属蛋白酶抑制剂-1 自发性气胸 肺大疱 

分 类 号：R561.4[医药卫生—呼吸系统]