罗非鱼类立克次氏体病的组织病理学观察  

Histopathological Observation of Disease of Rickettsia-like Organism in Tilapia(Oreochromis niloticus)

作　　者：戚瑞荣 唐绍林 崔龙波[3] 雷燕 马家好 

机构地区：[1]广州利洋水产科技股份有限公司,广东广州510515 [2]广州金水动物保健品有限公司,广东广州510515 [3]烟台大学生命科学学院,山东烟台264005

出　　处：《水产学杂志》2016年第3期21-24,共4页Chinese Journal of Fisheries

基　　金：国家科技支撑计划项目(2012BAD17B01)

摘　　要：本文主要研究感染类立克次氏体(Rickettsia-like organism,RLO)的养殖罗非鱼(Oreochromis aureus♂×Oreochromis niloticus♀)肝、脾、肾、肠、鳃和心脏的组织病理变化。感染类立克次氏体的罗非鱼各器官广布粗糙不圆整的白色小结节,这些结节为肉芽肿坏死灶,分层不典型,与周围健康组织的界限不甚明显;严重病变的组织坏死区域较大,与周围明显增生的细胞形成清晰的巨大"肉瘤"状肿物。经吉姆萨-瑞氏染色后,在病变组织内,尤其是脾肾组织的巨噬细胞胞质内可见蓝紫色球状或颗粒样、成簇分布的类立克次氏体。这些发现可为罗非鱼类立克次氏体病的病理学诊断提供依据。The histopathological changes in liver, spleen, kidney, intestine, gill and heart were described in tilapia （Oreochromis aureus × Oreochromis niloticus 9 ） infected by rickettsia-like organism （RLO）. The tilapia infected by RLO is most pathologically and anatomically characterized by rich in rough small white nodules without round in shape in diagnosis. The microscopic observation revealed that the nodules are the atypically stratified granuloma in structure without obvious boundaries between the necrosis tissues and healthy tissues. It appears that the nodules are obviously a huge ＂sarcoma＂ due to the large and obvious cell proliferation in the tissue of severe lesions. The RLO are bluish violet, spherical or particles and clustered, and observed in the lesion in tissue imprint stained by Giemsa-Wright, especially in the macrophages of spleen and kidney. The findings can provide evidence for pathologic diagnosis of the disease of rickettsia-like organism in tilapia.

关 键 词：类立克次氏体 罗非鱼 组织病理 组织印片 吉瑞染色 

分 类 号：S941.42[农业科学—水产养殖]