5-脂氧合酶抑制剂对人肝癌细胞株HepG2体外迁移及侵袭的影响  

Impact of 5-lipoxygenase inhibitors in human hepatic carcioma cell line HepG2 on the migration and invasion in vitro

作　　者：张强[1] 

机构地区：[1]河南省焦作市人民医院肿瘤外科,河南焦作454150

出　　处：《中国现代普通外科进展》2016年第5期347-350,354,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :观察5-脂氧合酶(5-LOX)抑制剂对人肝癌细胞株HepG2体外迁移及侵袭的影响及其部分机制的探析。方法:将不同浓度5-LOX抑制剂体外培养人肝癌HepG2细胞,通过细胞迁移划痕愈合实验、侵袭实验法检测5-LOX抑制剂对细胞体外迁移、侵袭抑制的情况,同时利用Western blot法检测不同浓度5-LOX抑制剂干预后人肝癌HepG2细胞MMP-9蛋白的表达情况。结果:5-LOX在细胞质中表达,部分在细胞核也有表达,人正常肝细胞5-LOX弱阳性,肝癌细胞HepG2中5-LOX呈强阳性表达;5-LOX抑制剂各组各个时相点的划痕愈合率均显著低于空白对照组(P<0.05),并且随着5-LOX浓度的增加降低的趋势逐渐明显;随着5-LOX抑制剂浓度的上调,对人肝癌HepG2细胞的抑制率逐渐上调;不同浓度5-LOX抑制剂可抑制MMP-9蛋白的表达,并随着浓度逐渐上调抑制作用更加明显。结论:5-LOX抑制剂可抑制人肝癌细胞株HepG2迁移及侵袭,这一过程可能与下调细胞MMP-9蛋白的表达有关。Objective： to observe the impact of 5-lipoxygenase （5-LOX） inhibitors in human hepatic carcioma cell line HepG2 on migration and invasion in vitro, and to explore the part of the mechanism. Methods： different concentrations of 5-LOX inhibitors were applied in vitro on human hepatic carcioma cell line HepG2 cells, detected the cell migration and invasion inhibition in vitro of 5-LOX inhibitors by the cell migration scratches healing experiment and invasive assay, simultane ously using Western blot method to test the protein xpression of MMP-9 on human hepatic car- cioma cell line HepG2 cells after different 5-LOX inhibitors intervention. Results： 5-LOX expressed in cytoplasm, and part of 5-LOX also expressed in the cell nucleus.People have weak positive 5-LOX in the normal liver cells, liver cancer cells HepG2 presented strong positive expression of 5-LOX. Scratches healing rates of 5-LOX inhibitors in each groups in all time points were significantly lower than the blank control group（P〈 0.05）, the trend of reduction gradually the more obvious with the increase of concentration of 5-LOX. With the increase of the concentration of LOX-5 inhibitors, the inhibition rate of human hepatic carcioma cell HepG2 increased gradually. Different 5-LOX inhibitors could inhibit the protein expression of MMP-9, and more apparent inhibitory effect with the gradually increased concentration. Conclusion： 5-LOX inhibitors can inhibit the accidental migration and invasion of human hepatic carcioma cell line HepG2, this process may be related to reduce the protein expression of MMP- 9.

关 键 词：5-LOX 抑制剂 人肝癌 HEPG2细胞株 体外迁移 侵袭 MMP-9 

分 类 号：R34[医药卫生—基础医学] R735.7