SOST和β-catenin在不同分期膝骨关节炎患者软骨及软骨下骨表达的研究  

The expression of SOST and β-catenin in the cartilage and subchondral bone of osteoarthritis patients at different stage

作　　者：吴疆[1] 吴龙[1] 马龙[1] 金群华[1] 

机构地区：[1]宁夏医科大学总医院骨三科,银川750004

出　　处：《中国骨质疏松杂志》2016年第6期689-694,共6页Chinese Journal of Osteoporosis

摘　　要：目的通过测定β-catenin和骨硬化蛋白(SOST)在不同分期人膝关节骨关节炎软骨、软骨下骨中的表达变化情况,探讨Wnt-β-catenin信号通路在骨关节炎患者的疾病进展中起到的作用。方法所有的骨关节炎标本均取自因膝关节骨关节炎合并内翻屈曲畸形行全膝人工关节置换术(TKA)的患者自愿捐赠的新鲜内侧胫骨平台组织,共39例。正常的软骨及软骨下骨组织均取自因下肢毁损伤行下肢截肢术的患者所捐赠的新鲜内侧胫骨平台组织,共6例。切片后用番红O固绿染色,采用改良Mankin评分将所有标本分为正常组,中度OA组和重度OA组。用免疫组化方法检测这3组中软骨及软骨下骨中β-catenin和SOST的表达情况。结果根据改良Mankin评分结果,45例标本中,6例为正常,14例为中度OA,25例为重度OA。β-catenin在软骨和软骨下骨中表达均持续升高,软骨中染色阳性细胞数量百分比在正常组,中度OA组和重度OA组中分别为17%,49%和62%,软骨下骨中分别为42%,59%和74%。而软骨中SOST染色阳性细胞百分比在则在中度OA组中表达量最高(59%),在正常组(11%)和重度OA组(12%)中表达量均明显少于中度组。在软骨下骨中,SOST染色阳性细胞百分比正常组中为58%,中度OA组为38%,重度OA组为16%,其表达量持续降低。结论β-catenin及SOST与骨关节炎疾病的发展及严重程度有关,甚至影响到Wnt-β-catenin信号通路,其可能在骨关节炎疾病的发展中起到重要作用。Objective To determine the effect of Wnt-β-catenin pathway on the process of osteoarthritis（ OA） by quantifying the expression of β-catenin and sclerostin（ SOST） in the cartilage and subchondral bone in OA patients at different disease stage.Methods All the cartilage and subchondral bone samples were obtained from OA patients undergoing total knee arthroplasty（ TKA,n = 39） and patients undergoing amputation due to lower extremity injury（ n = 6）. Cartilage and adjacent subchondral bone isolated from medial tibial plateau were stained with Safranin O. Modified Mankin score was used for classified the stage of the samples. The samples were then divided into normal group,mid-stage OA group,and end-stage OA group. Immunohistochemistry was used to quantify the expression of β-catenin and SOST in the 3 groups. Results According to the modified Mankin score,6samples were normal,14 were mid-stage OA,and 25 samples were end-stage OA,of the 45 samples. The percentage of β-catenin staining positive cells in cartilage was 17% in normal group,49% in mid-stage OA group,and 62% in end-stage OA group,and in subchondral bone was 59% in mid-stage OA group,11% in normal group,and 12% in end-stage group,respectively. The percentage of SOST staining positive cells in the cartilage was the highest in mid-stage OA group（ 59%）,but less in normal group（ 11%） and end-stage OA group（ 12%）. The percentage of positive staining cells in subchondral bone was 58% in normal group,38% in mid-stage OA group,and 16% of end-stage OA group,respectively. Conclusion The expressions of β-catenin and SOST are associated with the development of OA,suggesting that Wnt-β-catenin signaling pathway plays an important role in the process of OA.

关 键 词：骨关节炎 软骨 软骨下骨 骨硬化蛋白 Wnt-β-catenin信号通路 

分 类 号：R684.3[医药卫生—骨科学]