左归丸、右归丸对去卵巢骨质疏松大鼠神经肽CGRP、SP、VIP、NPY的影响  

Effect of Zuogui and Yougui pills on neuropeptide CGRP,SP,VIP,and NPY in osteoporotic rats after ovariectomy

作　　者：杨锋[1] 杨利学[1] 李小群[1] 李彦民[1] 

机构地区：[1]陕西中医药大学附属医院,陕西咸阳712000

出　　处：《中国骨质疏松杂志》2016年第6期761-765,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金项目(81102610;81473711);陕西省中医管理局科研项目(13-JC014);陕西省科技计划项目(2015SF072)

摘　　要：目的观察补肾药左归丸、右归丸对去卵巢骨质疏松大鼠神经肽的影响,阐明补肾法治疗骨质疏松症的神经调节机制。方法建立去卵巢骨质疏松模型,分别采用ELISA、免疫组化以及PCR观察补肾药干预后骨组织及骨髓间充质干细胞中神经肽CGRP、SP、VIP、NPY的变化。结果切除卵巢后大鼠骨组织及骨髓间充质干细胞中神经肽CGRP、SP、VIP、NPY的水平明显下降,补肾药干预后CGRP、SP、VIP、NPY的水平明显上升,但仍低于假手术组。结论补肾药可以提高骨质疏松大鼠神经肽CGRP、SP、VIP、NPY,这可能是补肾药治疗骨质疏松症的机制之一。Objective To observe the effect of Zuogui and Yougui pills on the neuropeptides in ovariectomy-induced osteoporotic rats,and to clarify the neural regulation mechanism of kidney tonic medicine in the treatment of osteoporosis. Methods Osteoporosis model was established by ovariectomy. The change of neuropeptides CGRP,SP,VIP,and NPY in the bone and bone marrowmesenchymal stem cells was observed using ELISA,immunohistochemistry,and PCR method after kidney tonic medicine intervention. Results After ovariectomy,the levels of neuropeptides CGRP,SP,VIP,and NPY in the bone tissue and bone marrowmesenchymal stem cells declined obviously. After the intervention of kidney tonic medicine,the levels of CGRP,SP,VIP,and NPY increased obviously,but were still lower than those in sham operation group. Conclusion Kidney tonic medicine improves neuropeptides CGRP,SP,VIP,and NPY in osteoporotic rats. It may be one of the mechanisms of treating osteoporosis.

关 键 词：中医中药 骨质疏松 神经肽 

分 类 号：R274.9[医药卫生—中西医结合]