纤维素酶高产菌株的产酶条件优化  

Optimization of Enzyme Production Conditions for High-yield Cellulase Strains

作　　者：张铁涛[1] 徐云升[1] 

机构地区：[1]琼州学院食品学院,海南三亚572022

出　　处：《中国调味品》2016年第6期50-54,共5页China Condiment

基　　金：国家自然科学基金项目(21262026)

摘　　要：采用刚果红鉴别培养基,从倒伏或被砍伐的香蕉株附近的腐质土样中,通过筛选得到一株产纤维素酶活力高且稳定性良好的菌株A2,镜检初步断定菌株A2为真菌,确定最佳产酶条件为培养基初始pH 5.5,蛋白胨含量0.60%,碳源含量2.40%,此时滤纸片酶活法(FPA)测定酶活力最大为2.069IU/g。CMC-Congo red stain is used to screen the cellulase-degrading strain A2 with high cellulase activity and good stability from strain mentioned above is identi show that the initial pH of medi lodgy or felled banana plant near the humus soil sample. Then the fled as fungus by its morphology. The results of fermentation test um is 5.5, the content of peptone is 0.60%, the content of carbon source is 2.40%, and the maximal activity of FPA is 2. 069 IU/g.

关 键 词：鉴别培养基 纤维素酶 FPA 

分 类 号：TS201.25[轻工技术与工程—食品科学]