卷烟烟气暴露下AA549和BEAS-22B细胞促炎性因子的释放变化  

Release of proinflammatory cytokines from A549 cells and BEAS-2B cells exposed to mainstream cigarette smoke

作　　者：李翔[1] 张世敏 赵俊伟[1] 尚平平[1] 彭斌[1] 谢复炜[1] 刘惠民[1] 谢剑平[1] 

机构地区：[1]中国烟草总公司郑州烟草研究院 [2]平顶山市烟草专卖局(公司)

出　　处：《烟草科技》2016年第6期45-49,共5页Tobacco Science & Technology

基　　金：中国烟草总公司郑州烟草研究院科技项目"基于MRTP健康风险评估框架的体外毒理学测试方法研究"(322013CZ0600)

摘　　要：为比较不同类型细胞对卷烟烟气诱导的促炎症反应的应答差异,采用中性红细胞毒性试验测试了卷烟烟气总粒相物(TPM)和全烟气(WS)对人肺腺癌细胞A549和人支气管上皮细胞BEAS-2B的细胞毒性效应;采用酶联免疫法(ELISA)分析了TPM和WS暴露后,A549和BEAS-2B促炎性细胞因子白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的释放水平。结果表明:BEAS-2B细胞较A549细胞对卷烟烟气的细胞毒性效应敏感。卷烟烟气暴露下A549细胞没有增加促炎性细胞因子IL-6和IL-8的释放;而BEAS-2B细胞在TPM和WS暴露下其IL-6和IL-8的释放显著增加。卷烟烟气诱导的促炎症反应存在细胞类型的差异。In order to compare the differences of response to proinflammatory reaction induced by mainstream cigarette smoke among cells of different types, neutral red uptake（NRU） assay was employed to assess the cytotoxicity of cigarette smoke total particulate matter（TPM） or whole smoke（WS） to A549 cells（human adenocarcinoma cells） and BEAS-2B cells（human bronchial epithelial cells）. The levels of proinflammatory cytokines interleukin-6 and interleukin-8（IL-6 and IL-8） released from A549 and BEAS-2B cells exposed to TPM and WS were analyzed by ELISA. The results showed that BEAS-2B cells were more sensitive to smoke-induced cytotoxicity than A549 cells. The induction of IL-6 and IL-8 released from A549 cells was not impacted by cigarette smoke exposure, while TPM and WS all caused dose-dependently increase of IL-6 and IL-8 released from BEAS-2B cells. The proinflammatory response to cigarette smoke thus might be different depending on cell types.

关 键 词：卷烟烟气 全烟气暴露 烟气总粒相物 促炎性细胞因子 细胞毒性 

分 类 号：TS416[农业科学—烟草工业]