植物细胞固定化条件对海南粗榧细胞生产三尖杉酯碱的影响  

Effects of the Conditions for Immobilized Cell of Plant on the Harringonine Production of Cephalotaxus mannii Immobilized Cells

作　　者：潘亚华 蒋旋娴 李永成[1] 

机构地区：[1]海南大学食品学院,海南海口570228

出　　处：《海南大学学报（自然科学版）》2016年第2期155-160,共6页Natural Science Journal of Hainan University

基　　金：国家自然科学基金(NO.21166007)

摘　　要：为了研究海南粗榧细胞的固定化条件及其对细胞的生长和三尖杉酯碱合成的影响,本研究利用海藻酸钠凝胶包埋法对海南粗榧悬浮细胞进行固定化,并改变了其固定化条件,同时测定了生物量、PAL酶活性、凝胶球硬度、蔗糖含量和三尖杉酯碱产量等指标,结果显示：海南粗榧固定化细胞的生长周期为40 d,其中,在0~15 d其细胞生长停滞,在15~30 d其细胞生长缓慢,而30~40 d为缓慢衰亡期;海南粗榧细胞固定化培养的最佳产物提取时间为第15 d;包埋过程中Ca Cl_2的最适质量浓度为0.023 g/m L;鲜重细胞的最适接种量为10%.由此得出结论：海南粗榧细胞固定化培养抑制了细胞的生长,延长了细胞的生长周期,但缩短了细胞生产三尖杉酯碱的周期.In the study, to study the conditions for immobilizing cell of Cephalotaxus mannii and its effects on the growth and harringtonine synthesis of C. marmi, the sodium alginate embedding method was performed to im- mobilize C. mannii , the immobilized conditions were changed, and biomass, the activity of phenylalanine am- monium-lyse, the hardness of gel beads, sucrose content, and the production of harringtonine were determined. The results indicated that the growth cycle of the immobilized cell of C. mannii was 40d, the lag period of growth was during 0- 15d, the slow growing time was during 15 -30th days, and the stable phase was during 30 - 40d; the culture conditions of the immobilized cell of C. mannii was optimized as followed： the optimal time point to extract the product of C. mannii was the 15th day; the optimal concentration of CaCl2 was 0. 023 g. mL-1; to obtain the maximum harringtonine production, the optimal fresh weight of C. mannii was 10%. The culture conditions for immobilizing cell of C. mannii inhibited the growth and extended the growth time of C. mannii cell, but this method shortened processing periods of the harringtonine.

关 键 词：海南粗榧 固定化 海藻酸钠 三尖杉酯碱 

分 类 号：Q831.1[生物学]