生血宁片对环磷酰胺致贫血小鼠的作用及机制  

Effect of Shengxuening Tablets on mice with cyclophosphamide-induced anemia and its mechanism

作　　者：黄飞鸿[1] 朱林杰[1] 李迪迪[1] 于娅[1] 瞿礼萍[1] 邹文俊[1] 吴建明 

机构地区：[1]成都中医药大学药学院,四川成都616000 [2]西南医科大学药学院,四川泸州646000

出　　处：《中成药》2016年第6期1205-1210,共6页Chinese Traditional Patent Medicine

摘　　要：目的考察生血宁片(铁叶绿酸钠和叶绿素衍生物)对环磷酰胺致骨髓抑制性贫血小鼠的作用及其分子机制。方法体外实验培养正常小鼠及腹腔注射环磷酰胺小鼠的骨髓有核细胞(BMC),不同剂量的生血宁片处理这些BMC,采用ATP生物发光法测定生血宁片对其BMC增殖。体内实验,采用腹腔注射环磷酰胺复制贫血模型,以低、中、高剂量的生血宁片进行治疗,给药后第7、10、13天测定外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、红细胞压积(HCT)水平,第10天收集小鼠骨髓细胞,检测其白介素(Il3)、粒细胞-巨噬细胞集落刺激因子(Gm-csf)、干细胞因子(Scf)mRNA表达水平。结果体外实验显示,生血宁片显著提高两类BMC的增殖率(P<0.05,P<0.01);体内实验显示,生血宁片500、353 mg/kg组可显著升高小鼠外周血WBC、RBC、HGB、HCT水平(P<0.05,P<0.01),生血宁片500、353、250 mg/kg组可显著升高骨髓细胞Il3、Scf mRNA表达水平(P<0.05,P<0.01)。结论生血宁片促进Scf和Il3 mRNA的表达,从而缓解环磷酰胺引起的骨髓抑制。AIM To investigate the effect of Shengxuening Tablets（sodium iron chlorophyllin,chlorophyll derivatives） on mice with cyclophosphamide-induced anemia and its mechanism of action.METHODS In vitro,the bone marrow mononeuclear cells（BMC） were isolated from the normal mice and myelosuppressive mice that have received intraperitoneal injection of cyclophosphamide.BMC obtained were treated with the different dose of Shengxuening Tablets for eleven days.The proliferation of BMC was measured by ATP bioluminescence assay.In vivo,anemia was established by intraperitoneal injection cyclophosphamide,the anemia mice was treated with the low-,middle-,and high-dose of Shengxuening Tablets for thirteen days.On the 7th,10 thand 13thday,the peripheral blood was collected from fundus venous plexus to count WBC,RBC,HGB and HCT.On the 10 thday,the bone marrows were collected to measure the mRNA expressions of Il3,Scf and Gm-csf by q PCR analysis.RESULTS In vitro,Shengxuening Tablets could significantly improve the proliferation of the normal mice BMC and that of myelosuppressive mice induced by cyclophosphamide（P〈0.05）.In vivo,the WBC,RBC,HGB and HCT in middle-,and high-dose of Shengxuening Tablets groups and the mRNA expressions of Il3,Scf in low-,middle-,and high-dose of Shengxuening Tablets groups were significantly increased,compared with those of the model group（P〈0.05）.CONCLUSION Shengxuening Tablets can alleviate cyclophosphamide-induced myelosuppressive anemia in mice.Its therapeutic mechanism is possibly related to the improvement of the mRNA expressions of Il3 and Scf.

关 键 词：生血宁片 骨髓抑制 环磷酰胺 贫血 mRNA表达 IL-3 SCF 

分 类 号：R285.5[医药卫生—中药学]