BP-ANN结合正交试验法优化白附子多糖提取工艺  

Optimizing the extraction of polysaccharides from Typhonii Rhizoma by BP-ANN combined with orthogonal test

作　　者：吴娜[1] 赵重博[1] 胡美变 彭伟[1] 解达帅 吴纯洁[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《中成药》2016年第6期1248-1253,共6页Chinese Traditional Patent Medicine

基　　金：四川省教育厅科技基础性工作专项分项目(2014FY111100-4)

摘　　要：目的采用反向传播人工神经网络(BP-ANN)结合正交试验法优化白附子多糖提取工艺。方法水提醇沉法提取多糖后,苯酚-硫酸法测定含有量。基于单因素试验的基础上,按照L9(34)正交表安排进行试验,考察提取温度、提取时间、料液比对多糖得率的影响,BP-ANN技术优化提取工艺。结果最佳条件为提取时间3.5 h,提取温度90℃,料液比1∶20,多糖得率达5.20%。结论该方法重复性良好,而且试验次数少。AIM To optimize the extraction of polysaccharides from Typhonii Rhizoma by back-propagation artificial neural network（BP-ANN） combined with orthogonal test.METHODS For polysaccharides extracted by aqueous extraction-alcohol precipitation technology,the content was determined by phenol-sulfuric acid method.Based on single factors experiments,the L9（3^4） orthogonal test was conducted to investigate the effects of extraction temperature,extraction time,and solid-liquid ratio on polysaccharides extraction yield.Subsequently,the extraction was optimized by BP-ANN.RESULTS Provided at a 5.20% high yield of polysaccharides,the best conditions were thus determined to be 90 ℃ for extraction temperature,3.5 h for extraction time,and 1 ∶ 20 for solid-liquid ratio.CONCLUSION The work proves that this method has good reproducibility and the advantage with fewer test times.

关 键 词：白附子 多糖 提取工艺 BP-ANN 正交试验 

分 类 号：R284.2[医药卫生—中药学]