UHPLC-MS/MS法同时测定蒲地蓝消炎片中10种成分  

Simultaneous determination of ten constituents in Pudilan Xiaoyan Tablets by UHPLC-MS/MS

作　　者：米建萍[1,2] 庞倩[2,3] 徐远金[2,3] 

机构地区：[1]北海市疾病预防控制中心,广西北海536000 [2]亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [3]广西大学化学化工学院,广西南宁530004

出　　处：《中成药》2016年第6期1269-1273,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(21264003)

摘　　要：目的建立超高效液相色谱-串联质谱（UHPLC-MS/MS）法同时测定蒲地蓝消炎片（蒲公英、黄芩、苦地丁和板蓝根）中腺苷、表告依春、咖啡酸、绿原酸、阿魏酸、紫堇灵、乙酰紫堇灵、芹菜素、黄芩素和汉黄芩素的含有量。方法分析采用Eclipse Plus C18RRHD色谱柱（1.8μm,2.1 mm×50 mm）;流动相为乙腈-0.2%乙酸水溶液,梯度洗脱;体积流量为0.3 m L/min。结果 10种成分分别在0.000 050 0～1.00、0.010 0～5.00、0.015 0～10.0、0.030 0～20.0、0.100～100、0.000 300～10.0、0.003 00～10.0、0.000 600～20.0、0.200～20.0、0.000 500～5.00 mg/L范围内线性关系良好,平均加样回收率在91.4%～105.0%之间,RSD均小于2.0%。结论该方法快速、简便、灵敏,适用于蒲地蓝消炎片的质量控制。AIM To establish an ultra performance liquid chromatography with tandem mass spectrometry（UHPLC-MS/MS） method for the simultaneous determination of adenosine,epigoitrin,caffeic acid,chlorogenic acid,ferulic acid,corynoline,acetylcorynoline,apiginin,baicalein and wogonin in Pudilan Xiaoyan Tablets（Taraxaci Herba,Scutellariae Radix,Corydalis Bungeanae Herba and Isatidis Radix）.METHODS The analysis was performed on an Eclipse Plus C18 RRHD column（1.8 μm,2.1 mm × 50 mm）,with a mobile phase comprising of acetonitrile-0.2% acetic acid aqueous solution in a gradient elution manner,at a flow rate of 0.3 m L/min.RESULTS Ten constituents showed good linear relationships within the ranges of 0.000 050 0-1.00 mg/L,0.010 0-5.00 mg/L,0.015 0-10.0 mg/L,0.030 0-20.0 mg/L,0.100-100 mg/L,0.000 300-10.0 mg/L,0.003 00-10.0 mg/L,0.000 600-20.0 mg/L,0.200-20.0 mg/L and 0.000 500-5.00 mg/L,respectively,whose average recoveries were 91.4%-105.0% with the RSDs of less than 2.0%.CONCLUSION This rapid,simple and sensitive method suits the quality control of Pudilan Xiaoyan Tablets.

关 键 词：蒲地蓝消炎片 化学成分 UHPLC-MS/MS 

分 类 号：R927.2[医药卫生—药学]