HPLC-DAD法和一测多评法同时测定安息香中4种成分  

Simultaneous determination of four constituents in Styrax tonkinensis by HPLCDAD and QAMS

作　　者：胡攀[1] 夏厚林[1] 文冰杰 李瑞煜[1] 杨孟妮 张慧[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川成都611137

出　　处：《中成药》2016年第6期1274-1278,共5页Chinese Traditional Patent Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB512606)

摘　　要：目的建立高效液相色谱-二极管阵列检测器（HPLC-DAD）法和一测多评（QAMS）法同时测定安息香Styrax tonkinensis（Pierre）Craib ex Hart.中香草醛、苯甲酸、苯甲酸松柏酯以及苯甲酸苄酯的含有量。方法 HPLC-DAD法同时测定4种成分含有量,计算苯甲酸松柏酯与其他3种成分的相对校正因子,考察相对保留时间法和线性回归法对色谱峰定位的影响,并比较这两种方法的重复性。结果 15批样品中香草醛、苯甲酸、苯甲酸松柏酯、苯甲酸苄酯的含有量分别为0.692%～2.621%、2.944%～15.628%、12.649%～62.589%、0.514%～2.034%,线性回归法较相对保留时间法更准确,3个相对校正因子重复性良好,两种方法无显著差异。结论一测多评法可用于测定安息香中的总香脂酸类成分。AIM To establish high performance liquid chromatography with diode array detection（HPLCDAD） and quantitative analysis of multi-components by single marker（QAMS） methods for the simultaneous determination of contents of vanillin,benzoic acid,coniferyl benzoate and benzyl benzoate in Styrax tonkinensis（Pierre）Craib ex Hart..METHODS HPLC-DAD was applied to simultaneously determining the contents of these four constituents.The relative correction factors of coniferyl benzoate to another three constituents were calculated.The effects of relative retention time and linear regression methods on positioning chromatographic peaks were investigated.And the reproducibilities were then compared.RESULTS In fifteen batches of samples,the contents of vanillin,benzoic acid,coniferyl benzoate and benzyl benzoate were 0.692%-2.621%,2.944%-15.628%,12.649%-62.589% and 0.514%-2.034%,respectively.Linear regression method showed its superioty in accuracy as compared with relative retention time method.The reproducibilities of three relative correction factors were good.And these two methods demonstrated no significant difference.CONCLUSION QAMS can be used for determining total balsamic acids in S.tonkinensis.

关 键 词：安息香 香草醛 苯甲酸 苯甲酸松柏酯 苯甲酸苄酯 HPLC-DAD 一测多评 

分 类 号：R284.1[医药卫生—中药学]