如达七味散质量标准的研究  

Quality standard for Ruda Qiwei Powder

作　　者：玉华[1] 

机构地区：[1]内蒙古民族大学,内蒙古通辽028000

出　　处：《中成药》2016年第6期1285-1288,共4页Chinese Traditional Patent Medicine

基　　金：内蒙古民族大学科研基金项目(NMD1335)

摘　　要：目的建立蒙药如达七味散（木香、栀子、荜茇等）的质量标准。方法 TLC法鉴别木香、栀子、荜茇。HPLC分析采用Inertsil C18色谱柱（150 mm×4.6 mm,5μm）;流动相甲醇-水（59∶41）;柱温30℃;体积流量1.0 m L/min;检测波长225 nm（木香烃内酯、去氢木香内酯）和343 nm（胡椒碱）。结果 TLC斑点清晰,专属性强。木香烃内酯、去氢木香内酯和胡椒碱分别在76.6～459.6 ng、123.6～741.6 ng、130.62～738.72 ng范围内线性关系良好,平均回收率分别为99.07%、98.87%、99.24%,RSD分别为1.02%、1.58%、1.76%（n=6）。结论该方法精密度高,重复性好,可用于如达七味散的质量控制。AIM To establish the quality standard for Mongolian medicine Ruda Qiuwei Powder（Aucklandiae Radix,Gardeniae Fructus,Piperis Longi Fructus,etc.）.METHODS TLC was applied to identifying A.Radix,G.Fructus and P.Longi.HPLC analysis was performed on a 30 ℃ thermostatic Inertsil C18column（150 mm ×4.6 mm,5 μm）,with the mobile phase comprising of methanol-water（59 ∶ 41） at a flow rate of 1.0 m L/min,and the detection wavelengths were set at 225 nm for costunolide and dehydrocostus lactone,and 343 nm for piperine.RESULTS The clear spots proved the specificity of TLC.Costunolide,dehydrocostus lactone and piperine showed good linear relationships within the ranges of 76.6-459.6 ng,123.6-741.6 ng and 130.62-738.72 ng.The average recoveries were 99.07%,98.87% and 99.24% with the RSDs of 1.02%,1.58% and 1.76%（n = 6）,respectively.CONCLUSION With high precision and good reproducibility,this method can be used for the quality control of Ruda Qiwei Powder.

关 键 词：如达七味散 木香 栀子 荜茇 木香烃内酯 去氢木香内酯 胡椒碱 

分 类 号：R927.2[医药卫生—药学]