复方茜草片质量标准的研究  

Quality standard for Compound Rubia Tablets

作　　者：方乐敏 杨骏[1,2] 刘云[1] 浦益琼[1] 张彤[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海市香山中医医院,上海200020

出　　处：《中成药》2016年第6期1293-1297,共5页Chinese Traditional Patent Medicine

基　　金：上海市科学技术委员会中药现代化专项(11DZ1971500);上海市卫生和计划生育委员会青年项目(20124Y002)

摘　　要：目的建立复方茜草片（茜草和新疆紫草）的质量标准。方法 TLC法对茜草和新疆紫草进行鉴别,HPLC法测定大叶茜草素含有量。分析采用C18色谱柱（250 mm×4.6 mm,5μm）;流动相甲醇-乙腈-0.2%磷酸（25∶50∶25）;体积流量1.0 m L/min;柱温25℃;检测波长250 nm。结果 TLC斑点清晰,分离度好。大叶茜草素在0.828 125～106μg/m L范围内线性关系良好（r=0.999 8）,平均加样回收率为96.66%（RSD=2.04%）。结论该方法可用于复方茜草片的质量控制。AIM To establish the quality standard for Compound Rubia Tablets（Rubia cordifolia L.and Arnebia euchroma [Royle] Johnst.）.METHODS R.cordifolia and A.Euchroma were identified by TLC,and the content of mollugin was determined by HPLC.The analysis was performed on a 25 ℃ thermostatic C18column（250 mm × 4.6 mm,5 μm）,with the mobile phase comprising of methanol-acetonitrile-0.2% phosphoric acid（25 ∶ 50 ∶ 25） at a flow rate of 1.0 m L/min,and detection wavelength was set at 250 nm.RESULTS The TLC spots were clear and well-separated.Mollugin showed a good linear relationship within the range of 0.828 125-106 μg/m L（r = 0.999 8）.The average recovery was 96.66%（RSD = 2.04%）.CONCLUSION This method can be used for the quality control of Compound Rubia Tablets.

关 键 词：复方茜草片 茜草 新疆紫草 大叶茜草素 

分 类 号：R927.2[医药卫生—药学]