灵芝子实体中的羊毛脂烷型灵芝酸酯类成分研究  

Lanostane-type ganoderic acids from the fruiting bodies of Ganodermalucidum

作　　者：杨玉 张宝璟[2] 霍晓奎[2] 邓卅[2] 张厚利[2] 马骁驰[2] 王超[2] 黄珊珊[2] 

机构地区：[1]大连市妇幼保健院药剂科,辽宁大连116033 [2]大连医科大学药学院,辽宁大连116044

出　　处：《中国微生态学杂志》2016年第6期645-649,共5页Chinese Journal of Microecology

基　　金：国家自然科学基金青年基金项目(81503201)

摘　　要：目的系统研究灵芝子实体中的羊毛脂烷型灵芝酸酯类成分。方法通过氯仿对盐酸酸化的灵芝子实体提取物中的羊毛脂烷型三萜酸类成分进行萃取,结合硅胶柱层析、中压柱层析(RP C18色谱柱)、制备型HPLC(RP C18色谱柱)对提取物中的三萜酸酯类化学成分进行系统的分离、纯化。通过UV、ESIMS、HRESI-MS、1 H-NMR、13C-NMR、HSQC、HMBC、NOESY等波谱学手段结合文献数据对制备得到的三萜类单体成分进行准确的结构测定。结果从灵芝子实体的氯仿提取物中共分离鉴定了10个羊毛脂烷型灵芝酸酯类成分,分别为:(1)Ganoderic acid AP,(2)Methyl Ganoderate G,(3)Ganoderic acid H,(4)12β-Hydroxy-3,7,11,15,23-pentaoxo-5α-lanost-8-en-26-oic acid,(5)Ganoderic acid K,(6)Ganoderenic acid B,(7)Lucienic acid A,(8)Lucidenic acid B,(9)20(21)-Dehydrolucidenic acid A,(10)Lucidone D。结论灵芝子实体中极性较小的化学成分主要为羊毛脂烷型三萜及其降碳衍生物,该类成分多具有羧基或羧酸酯官能团,既而也称灵芝酸类,本文共分离鉴定了10个该系列成分,其中化合物1为新化合物。Objective To study the lanostane-type ganoderic acids in the fruiting bodies of Ganodermalucidum（Polyporaceae）.Methods The lanostane-type triterpenoids in Ganodermalucidumfruiting bodies were extracted with CHCl3.The CHCl3 extract was separated by silica gel column chromatography and HPLC successively.On the basis of various spectroscopic data,including UV,ESI-MS,HRESI-MS,1 H-NMR,13 C-NMR and 2D-NMR,the structures of the isolated triterpenoids were elucidated.Results 10 lanostanetype ganoderic acids were obtained from the fruiting bodies of Ganodermalucidum,which were elucidated as Ganoderic acid AP（1）,Methyl Ganoderate G（2）,Ganoderic acid H（3）,12β-Hydroxy-3,7,11,15,23-pentaoxo-5α-lanost-8-en-26-oic acid（4）,Ganoderic acid K（5）,Ganoderenic acid B（6）,Lucienic acid A（7）,Lucidenic acid B（8）,20（21）-dehydrolucidenic acid A（9）,and Lucidone D（10）.Conclusion Ganoderic acid AP is a new triterpenoid.Lanostane-type triterpenoids were the major constituents of Ganodermalucidum.

关 键 词：灵芝 羊毛脂烷型三萜 灵芝酸 

分 类 号：R378.991[医药卫生—病原生物学]