盐胁迫对黄瓜幼苗叶片、韧皮部渗出液和根系抗氧化酶同工酶表达的影响  

NaCl stress on antioxidant enzyme isozymes expressed in cucumber seedling leaves, phloem exudates, and roots

作　　者：孙张晗 樊怀福[1,2] 杜长霞[1,2] 黄玲英[1] 

机构地区：[1]浙江农林大学浙江省农产品品质改良技术研究重点实验室,浙江临安311300 [2]浙江农林大学农业与食品科学学院,浙江临安311300

出　　处：《浙江农林大学学报》2016年第4期652-657,共6页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31201658;31101539);浙江省自然科学基金资助项目(LY15C150006;Y3110308);浙江农林大学科研发展基金人才启动项目(2011FR018)

摘　　要：黄瓜Cucumis sativus在设施生产中经常受到盐害,严重影响了黄瓜的产量和品质。以盐敏感黄瓜品种‘津优1号’Cucumis sativus‘Jinyou No.1’和相对较耐盐品种‘新泰密刺’Cucumis sativus‘Xintai Mici’为试材,采用水培,研究了氯化钠胁迫对幼苗叶片、根系和韧皮部渗出液超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)同工酶表达影响。结果显示:叶片中共检测到9条超氧化物歧化酶,4条过氧化物酶和2条过氧化氢酶同工酶条带,盐胁迫抑制了‘新泰密刺’叶片超氧化物歧化酶、过氧化物酶和过氧化氢酶同工酶表达,而‘津优1号’叶片超氧化物歧化酶和过氧化氢酶同工酶表达增强。韧皮部渗出液有3条超氧化物歧化酶,2条过氧化物酶和1条过氧化氢酶同工酶条带;盐胁迫增强了‘新泰密刺’韧皮部渗出液中3种抗氧化酶同工酶表达,而抑制了‘津优1号’韧皮部渗出液中3种同工酶表达。在根系中共发现6条超氧化物歧化酶,7条过氧化物酶和1条过氧化氢酶同工酶条带,盐胁迫下2个品种根系过氧化氢酶同工酶的表达均增强,过氧化物酶同工酶在‘津优1号’中6条表达减弱,1条增强,在‘新泰密刺’中均增强,2个品种中3条相同超氧化物歧化酶同工酶在盐胁迫下表达减弱,2条在‘津优1号’中表达增强而‘新泰密刺’中无变化,1条在‘津优1号’中表达无变化而‘新泰密刺’中表达减弱。综上所述,3种抗氧化酶同工酶与其耐盐性均有密切关系,并暗示抗氧化酶同工酶响应盐胁迫的变化趋势具有品种及组织特异性。韧皮部渗出液中3种同工酶变化趋势在2个品种中完全相反,说明韧皮部是黄瓜幼苗响应盐胁迫的重要组织。Cucumis sativus(cucumber) is frequently subjected to salinity in facility production, which seriously affects cucumber yield and quality. To determine the effect of Na Cl stress on cucumber, the salt-sensitive cultivar Cucumis sativus ‘Jinyou No. 1' and relative salt-tolerant cultivar Cucumis sativus ‘Xintai Mici' were used as experimental materials in this experiment. Seedlings were cultivated hydroponically with superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) isozyme expression being tested in cucumber seedling leaves, phloem exudates, and roots. Results for the leaves showed nine SOD isozyme bands, four POD isozyme bands, and two CAT isozyme bands being detected. With Na Cl stress in ‘Xintai Mici' leaves, SOD, POD, and CAT isozymes were inhibited, but in ‘Jinyou No. 1' leaves they were enhanced. In phloem exudates, isozyme bands of three SOD, two POD, and one CAT were detected with Na Cl stress enhanced in exudates of ‘Xintai Mici', but inhibited in ‘Jinyou No. 1'. In roots, isozyme bands from six SOD, seven POD, and one CAT were detected. The CAT isozyme expression in the roots of the two cultivars were enhanced; the six POD isozyme bands in ‘Jinyou No. 1' were enhanced, and one POD isozyme band was inhibited, but in ‘Xintai Mici' the expression of all POD isozyme bands was enhanced. With Na Cl stress three SOD isozymes were inhibited in both varieties, two SOD isozyme bands were enhanced in the ‘Jinyou No. 1' and they had no change in ‘Xintai Mici', and one SOD isozyme band had no change in ‘Jinyou No. 1' and its expression in the ‘Xintai Mici'was inhibited. Thus, the three antioxidant enzymes were related to salt tolerance to some extent implying that the antioxidant enzyme response to salt stress possessed variety and tissue specificity; whereas, changes in the three isozymes for phloem exudates were completely opposite in two cultivars suggesting that the phloem was an important tissue in response to salt stress of cucumber seedlings.

关 键 词：植物学 黄瓜 抗氧化酶同工酶 盐胁迫 

分 类 号：S642.2[农业科学—蔬菜学]