盖杨叶片高频植株再生体系建立  

Establishment of a High Efficient Plant Regeneration System of Populus gaixianensis

作　　者：冯连荣 

机构地区：[1]辽宁省杨树研究所,辽宁盖州115213

出　　处：《安徽农业科学》2016年第11期209-211,共3页Journal of Anhui Agricultural Sciences

基　　金：辽宁省科学事业公益研究基金项目(2014002016)

摘　　要：[目的]利用生物技术手段提高盖杨抗寒性并扩大其栽培区域。[方法]以盖杨叶片为外植体,研究不同激素组合对盖杨叶片分化及生根的影响,建立盖杨叶片高频植株再生体系。[结果]盖杨不定芽分化最适培养基配方为MS+6-BA 0.5 mg/L+NAA 0.20 mg/L+蔗糖25 g/L+琼脂6.0 g/L,p H 6.5,不定芽再生频率为96.67%,平均分化芽数20.3个;最适生根培养基配方为1/2MS+IBA0.3 mg/L+蔗糖20 g/L+琼脂6.0 g/L,p H 6.0,生根率可达100%,平均生根数15.8条。[结论]该研究建立了盖杨高效组织培养再生系统,为该杨树品种的快速无性繁殖和基因的遗传转化提供了依据。[Objective] The aim was to improve the cold resistance and expand its cultivation area of Populus gaixianensis by means of biotechnology.[Method] Leaves of P.gaixianensis were used as explants to study the effect of different hormone combinations on leaf differentiation and rooting,and establish a high efficient plant regeneration system.[Result] The results showed that the optimum medium for adventitious bud regeneration was MS ＋6-BA 0.5 mg/L ＋ NAA 0.20 mg/L ＋ sucrose 25 g/L ＋ agar 6.0 g/L,pH 6.5,with the frequency of adventitious bud regeneration of 96.67％ and the number of shoots of 20.3;the optimum medium for rooting was 1/2MS ＋ IBA 0.3 mg/L ＋ sucrose 20 g/L ＋ agar 6.0 g/L,pH 6.0,the rooting rate could reach 100％,and the average number of rooting was 15.8.[Conclusion] The study establishes high efficient tissue culture regeneration system for P.gaixianensis,lays a foundation for rapid vegetative propagation and genetic transformation of genes.

关 键 词：盖杨 叶片 再生体系 

分 类 号：S723.1[农业科学—林木遗传育种]