模拟微重力对猕猴脾脏结构、脾脏免疫细胞及其细胞因子表达的影响  

Effects of simulated microgravity on the structure, immune cell and cytokine expression of the spleen in rhesus monkey

作　　者：陈杨[1] 王萍[1] 许崇玉 蔡艺灵[2] 马华松[3] 

机构地区：[1]解放军306医院呼吸内科,北京100101 [2]解放军306医院神经内科,北京100101 [3]解放军306医院骨科,北京100101

出　　处：《解放军医学杂志》2016年第6期466-471,共6页Medical Journal of Chinese People's Liberation Army

基　　金：306医院院级青年基金课题(14QN04)~~

摘　　要：目的探讨模拟微重力对猕猴脾脏结构及免疫细胞表面分子和相关细胞因子表达的影响。方法在特殊装置上将猕猴置于头低位–10°以模拟微重力。将15只猕猴随机均分为对照组(NC组)、模拟微重力组(SM组)和模拟微重力后恢复组(MR组),每组5只。完成实验后,猕猴在麻醉状态下放血处死,取脾脏组织标本进行HE和免疫组化染色。观察各组猕猴脾脏大体结构,检测脾脏免疫细胞表面分子CD3、CD4、CD8、CD20、CD68及相关细胞因子(IL-1β、IL-5、IL-6、IL-17、IL-18、IL-22、IL-23)的表达。结果 NC组脾脏组织结构正常,SM组脾脏红、白髓分界不清,白髓缩小,动脉周围淋巴鞘减小,淋巴细胞排列紊乱,MR组改变较SM组轻。免疫组化结果显示,与NC组和MR组比较,SM组脾脏表达CD3+、CD4+、CD20+的免疫细胞数目降低,CD8+免疫细胞数目增高,IL-5分泌水平降低,差异有统计学意义(P<0.05)。结论模拟微重力可引起猕猴脾脏组织结构损伤,对CD3+T细胞、CD4+T细胞、CD8+T细胞和CD20+B细胞数目有一定影响,并能影响细胞因子IL-5分泌,这可能与模拟微重力引起免疫系统功能受损有关。Objective To investigate the effects of simulated microgravity on the structure, expression of surface molecules of immune cells and the expression of cytokines in the spleen of rhesus monkey. Methods Fifteen rhesus monkeys were employed as the research objects, and randomly assigned into three groups（5 each）： normal control（NC） group, simulated microgravity（SM） group and microgravity recovery（MR） group. A posture with head down for –10° was set for monkeys to simulate microgravity. All the rhesus monkeys were sacrificed under anesthesia after experiments completion. Spleen tissues were harvested and HE and immunohistochemically stained to observe the general structure of the spleen tissues in each group and detect the expressions of surface molecules（CD3, CD4, CD8, CD20 and CD68） in immune cells, and the expressions of related cytokines（IL-1, IL-5, IL-6, IL-17, IL-18, IL-22 and IL-23） on the splenic immune cells. Results Compared with the normal structure of spleen tissues in NC group, the dividing lines between red and white pulp were not clear, the white pulp and the periarteriolar lymphoid sheath were decreased, and the lymphocytes arranged disorder in SM group. The expressions of CD3, CD4 and CD20 decreased and of CD8 increased significantly in SM group（P〈0.05）. The production of IL-5 was significantly lower in SM group than in NC and MR groups（P〈0.05）. Conclusions Simulated microgravity may cause spleen tissue damage and throw an influence on the quantity of CD3^＋T-cell, CD4^＋ T-cell, CD8^＋ T-cell and CD20^＋ B-cell in rhesus monkey. Homogeneously, the production of IL-5 is decreased in spleen of rhesus monkey under simulated microgravity. These effects may be related to the damage of immune system under simulated microgravity.

关 键 词：模拟微重力 猕猴 脾脏 免疫细胞 细胞因子 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]