碳酸氢铵溶液增效高强度聚焦超声消融新鲜离体牛肝的实验研究  

Study on ammonium bicarbonate improving high intensity focused ultrasound efficiency for ablating bovine liver in vitro

作　　者：冯罡[1] 夏纪筑[1] 许春燕[1] 王志刚[1] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆市400010

出　　处：《临床超声医学杂志》2016年第6期361-365,共5页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金重大项目(81227801);国家自然科学基金青年项目(81501481)

摘　　要：目的研究碳酸氢铵溶液增效高强度聚焦超声(HIFU)消融离体牛肝的作用。方法以不同功率(120 W、150 W、180 W、210 W)HIFU消融离体牛肝,比较注射碳酸氢铵溶液的实验组与注射PBS溶液的对照组的凝固性坏死体积、灰度差及灰度区域面积。结果 HIFU消融功率为120 W时,实验组出现凝固性坏死,体积约(11.53±4.93)mm^3,而对照组无明显凝固性坏死;功率为150 W时,实验组凝固性坏死体积为(50.41±33.7)mm^3,显著大于对照组[(8.60±4.14)mm^3],差异有统计学意义(P<0.05);消融功率为180 W时,实验组凝固性坏死体积与对照组比较差异无统计学意义;消融功率为210 W时,实验组凝固性坏死体积为(39.84±13.62)mm^3,显著小于对照组[(62.79±11.32)mm^3],差异有统计学意义(P<0.05)。当HIFU消融功率为120 W、150 W时,实验组灰度差和灰度区域面积均显著高于对照组,差异均有统计学意义(均P<0.05)。结论在功率120 W、150 W时,碳酸氢铵溶液具备增效HIFU消融离体牛肝的作用。Objective To study the effect of ammonium bicarbonate solution on improving high intensity focused ultrasound（HIFU） efficiency to ablate bovine liver in vitro. Methods After ablating bovine liver in vitro with HIFU under different power levels（120 W,150 W,180 W,210 W）,the volumes of coagulative necrosis,gray scale difference and gray area of the experimental group with ammonium bicarbonate solution injection and the PBS-injected control group were measured.Results When HIFU ablation power was 120 W,the volumes of coagulation necrosis was（11.53±4.93）mm^3 in experimental group,while no coagulation necrosis in control group. When power was 150 W,the volumes of coagulation necrosis was（50.41±33.7）mm^3in experimental group,which was larger than that in control group[（8.60 ±4.14）mm^3],there was significant difference（P〈0.05）.When power was 180 W,there was no significant difference between the experimental group[（22.70±12.15）mm^3]and the control group[（15.47 ±6.48）mm^3]. However,the volumes of the coagulation necrosis in experimental group[（39.84±13.62）mm^3]was obviously smaller than that in control group[（62.79±11.32）mm^3]when power was 210 W,there was significant difference（P〈0.05）. When HIFU ablation power were 120 W and 150 W,the gray scale difference and gray area in experimental group were obviously higher than those in control group,there were significant difference（all P〈0.05）. Conclusion With appropriate power（120 W,150 W）,ammonium bicarbonate solution is able to assist HIFU in ablating bovine liver in vitro.

关 键 词：高强度聚焦超声 碳酸氢铵溶液 消融 牛肝 离体 

分 类 号：R332[医药卫生—人体生理学] R445.1[医药卫生—基础医学]