2株山西IBV分离株的生物学特性研究  

Biological characteristics of two strains of Shanxi IBV isolates

作　　者：高诗敏[1] 邓瑞林[1,2] 闫芳[1] 马海利[1] 化丽珍[1] 田文霞[1] 高文伟[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]南皮温氏畜牧有限公司,河北南皮061500

出　　处：《山西农业大学学报（自然科学版）》2016年第7期472-476,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西农业大学科技创新基金(412579)

摘　　要：[目的]控制地方鸡传染性支气管炎病的流行。[方法]通过IBV-21、IBV-22半数感染量(EID50)试验,鸡胚矮小化试验,干扰NDV LaSota复制试验,鸡胚气管环试验测定2毒株生物学特性。[结果]试验结果显示IBV-21的EID50为5×10-4.616·mL-1、IBV-22为5×10-6.5·mL-1;接毒胚明显病变,2株毒对鸡胚均有矮小化作用,对NDV LaSota有明显干扰,对鸡胚气管环试验病变明显,RT-PCR的方法扩增2分离株的S1基因的特异条带大小约为293bp。[结论]该生物学特性显示分离的2株病毒对鸡胚有明显致病作用且2毒株不是现存疫苗毒,为地方IBV疫苗研发奠定基础。[Objective]The aim was to control the epidemic of infectious bronchitis. FMethods]The biological characteris- tics of IBV-21 and IBV-22 were tested by infection of half quantity （EID50）, chicken embryos small test and interfer- ence NDV LaSota copy test and chicken embryo TOCs experiment. We verified by RT-PCR amplification. [Results] The results showed that the EID50 of IBV-21 was 5×10-4.616·mL-1、IBV-22was5×10-6.5·mL-1. Two strains had poison to chicken embryos that to be a little change as a result, the NDV LaSota interfered markedly, and chicken em- bryo TOCs was showed the lesions. The RT-PCR amplified S1 gene of 2 isolates, and stripe size was about 293 bp. [Conclusion]The biological characteristics showed that the separation of two viruses which was not existing vaccines bad obvious pathogenic role in chicken embryos, and laid the foundation in local IBV vaccine development.

关 键 词：IBV 生物学特性 RT-PCR 分离鉴定 

分 类 号：S855.3[农业科学—临床兽医学]