UVA诱导人皮肤成纤维细胞光老化模型中miR-222的调节作用  

RegulationEffects of miR-222 on UVA Irradiation-induced Photoaging of Human Dermal Fibroblasts

作　　者：宋健文[1] 吴凌[1] 宋建驷[2] 王博[3] 宫惠琳[4] 

机构地区：[1]西安市儿童医院皮肤科,陕西西安710003 [2]陕西科技大学体育部,陕西西安710021 [3]西安交通大学第一附属医院转化医学中心,陕西西安710061 [4]西安交通大学第一附属医院病理科,陕西西安710061

出　　处：《中国皮肤性病学杂志》2016年第7期674-677,共4页The Chinese Journal of Dermatovenereology

基　　金：陕西省自然科学基础研究计划项目(2013JM4060);陕西科技厅社会攻关项目(2014k11-05-03)

摘　　要：目的探讨miR-222在长波紫外线(UVA)诱导的光老化模型中的调节作用。方法分离并培养人皮肤成纤维细胞(HDFs),用0,2.5,5.0和7.5J不同剂量UVA辐射诱导光老化模型,用实时定量PCR和Western-blot分别检测miR-222和基质金属蛋白酶1(MMP1)的表达情况。用miR-222的功能模拟物和miR-222的功能抑制物分别转染HDFs,检测MMP1表达情况。对HDFs细胞分别转染miR-222功能模拟物、miR-222阴性对照物、miR-222功能抑制物和miR对照的功能抑制物,转染24h后予UVA辐射诱导光老化模型,检测MMP1表达情况。结果 HDFs经不同剂量UVA辐射后,MMP1表达与对照组相比显著升高(P<0.01),同时miR-222表达与对照组相比显著降低(P<0.01)。miR-222转染HDFs后再予UVA辐射,miR-222转染组MMP1表达显著低于miR对照组(t=5.616,P<0.05),而miR-222抑制物转染组MMP1表达与miR对照组相比无显著的改变。结论人皮肤成纤维细胞光老化模型中UVA辐射可导致miR-222表达降低,从而上调MMP1表达。Objective To investigate the effects of miR-222 on ultraviolet A （UVA） induced skin photoaging. Methods The primary human dermal fibroblasts （HDFs） were isolated and cultured. After exposure to different does （0, 2. 5, 5.0 and 7.5 J/cm2） of UVA, the mRNA and protein levels of metal matrix proteinase l （ MMP1 ） in HDFs were detected by real-time PCR and Western blot respectively, and the levels of miR-222 was detected by real-time PCR. MiR-222 mimic, miR mimic control, anti- miR-222 and anti- miR mimic control were respectively transfected into HDFs before UVA exposure, and then the protein levels of MMP1 were detected. Results After exposure to UVA, the expression of MMP1 was significantly increased （P 〈 0. 01 ）, while the levels of miR-222 was significantly decreased （P 〈 0. 01 ）. When HDFs was exposed to UVA after transfected with miR-222 mimic, the protein levels of MMP1 in miR-222 mimic transfected group was significantly lower than in miR control （ P 〈 0. 05 ） , while there was no difference between anti- miR-222 transfected group and control group. Conclusion UVA could down-regulate the levels of miR-222 thus down-regulating the expression of MMP1 in HDFs photoaging model.

关 键 词：长波紫外线 光老化 人皮肤成纤维细胞 MIR-222 基质金属蛋白酶-1 

分 类 号：R751[医药卫生—皮肤病学与性病学]