藏族药高原唐松草的质量标准  

Quality Standard of Thalictrum cultratum

作　　者：翟红伟[1,2] 潘晓鹃[2] 舒光明[2] 周先建[2] 杨新新[2,3] 张美[2] 

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]四川省中医药科学院,成都610041 [3]广东药科大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2016年第13期34-39,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省食品药品医疗器械市场整治及综合监管专项(B-2013-3-1-27)

摘　　要：目的：对藏族药马尾连及高原唐松草的基原、资源分布进行本草及文献资料的考证,并建立高原唐松草药材的质量标准。方法：采用显微方法对高原唐松草药材粉末进行鉴别;以盐酸小檗碱为对照品,采用TLC进行定性鉴别;参照《中国药典》2015年版四部通则相关方法对10批高原唐松草药材的水分、总灰分、酸不溶性灰分、醇溶性浸出物进行测定;采用HPLC测定盐酸小檗碱的含量。结果：高原唐松草药材粉末的显微鉴别特征性强;TLC斑点清晰,分离度好;10批药材的水分9.8%-11.7%;总灰分2.9%-5.2%;酸不溶性灰分0.6%-2.2%;醇溶性浸出物25.4%-33.7%。盐酸小檗碱在1.572-47.16 mg·L^-1呈良好的线性关系,平均回收率98.26%,RSD 2.2%,10批高原唐松草含盐酸小檗碱的质量分数0.01%-0.61%。结论：建立的方法操作简便、准确可靠、专属性及重复性均较好,可用于高原唐松草药材的质量控制。Objective： To study on the original,resource distribution of Rhizoma et Radix Thalictri and Thalictrum cultratum by herbalogical study and textual research,and to establish quality standard of T. cultratum.Method： Microscopical characteristics of T. cultratum powder were investigated. Taking berberine hydrochloride as the marker component,qualitative identification of sample was identified by TLC. According to methods in the2015 edition of Chinese Pharmacopoeia,the content of moisture,total ash,acid insoluble ash and alcohol soluble extractive from 10 batches of T. cultratum were determined. The content of berberine hydrochloride was determined by HPLC. Result： T. cultratum had good microscopic characteristics. TLC showed the spots developed clearly with good resolution. The content of moisture,total ash,acid insoluble ash and alcohol soluble extractive from samples were 9. 8%-11. 7%,2. 9%-5. 2%,0. 6%-2. 2% and 25. 4%-33. 7%,respectively. The concentration of berberine hydrochloride showed a good linear relationship in the range of 1. 572- 47. 16 mg · L^- 1,and the average recovery was 98. 26% with RSD of 2. 2%,the content of berberine hydrochloride in samples was 0. 01%-0. 61%. Conclusion： These established methods are accurate with strong specificity and good reproducibility,which can be used for quality control of T. cultratum.

关 键 词：藏族药 马尾连 高原唐松草 质量标准 薄层鉴别 盐酸小檗碱 

分 类 号：R284.1[医药卫生—中药学] R282.5[医药卫生—中医学]