潜阳育阴颗粒对自发性高血压大鼠肾脏氧化应激及炎症因子的影响  

Effects of Qianyang Yuyin Granules on Oxidative Stress and Inflammation Factors in Kidney of Spontaneously Hypertensive Rats

作　　者：方祝元[1] 丁康[2] 沈竹阳 蒋卫民[1] 

机构地区：[1]江苏省中医院,南京210029 [2]南京市中医院,南京210001 [3]江苏省中西医结合医院,南京210028

出　　处：《中国实验方剂学杂志》2016年第13期86-91,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81273713);教育部博士点专项课题项目(20123237110005)

摘　　要：目的:研究潜阳育阴颗粒对自发性高血压大鼠肾脏氧化应激及炎症因子的影响,并探讨其改善自发性高血压的作用机制。方法:自发性高血压大鼠,随机分为模型组、潜阳育阴颗粒组、贝那普利组及潜阳育阴颗粒+贝那普利组,分别给予潜阳育阴颗粒混悬液(5 g·kg-1),贝那普利混悬液(1.67 mg·kg-1),潜阳育阴颗粒混悬液(5 g·kg-1)+贝那普利混悬液(1.67 mg·kg-1)ig,另设正常血压(WKY)大鼠为正常组,连续给等容量药8周后处死大鼠收集肾脏组织。观察大鼠血压、微量白蛋白和肌酐的变化,蛋白质免疫印迹(Western blot)检测肾脏局部NADPH氧化酶-4(NOX4)的水平,核转录因子-κB(NF-κB)p65及肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6)蛋白表达量的变化,并观察潜阳育阴颗粒对这些指标释放量的影响。结果:与正常组比较,模型组大鼠血压、微量白蛋白和肌肝明显升高,肾脏组织NOX4,NF-κB,TNF-α和IL-6蛋白表达明显升高(P<0.01);潜阳育阴颗粒能抑制模型组肾脏NOX4的表达,降低NF-κB p65,TNF-α,IL-6蛋白表达量,降低血压、微量白蛋白和肌肝的含量(P<0.01),而潜阳育阴颗粒和贝纳普利两药协同的效果更加明显(P<0.05)。结论:潜阳育阴颗粒改善自发性高血压大鼠肾损害机制可能与抑制氧化应激进而减少炎症因子释放相关。Objective： To investigate the effects of Qianyang Yuyin granules（ QYYYG） on oxidative stress and inflammation factors in kidney of spontaneously hypertensive rats（ SHRs）. Method： SHRs were randomly divided into model group,QYYYG group,Benazepril group,and QYYYG ＋ Benazepril group. The rats received QYYYG suspension（ 5 g·kg-（-1））,Benazepril suspension（ 1. 67 mg·kg-（-1））,QYYYG suspension（ 5 g·kg-（-1）） ＋ Benazepril suspension（ 1. 67 mg·kg-（-1）） respectively by intragastric administration. WKY rats were used as normal group,and their renal tissues were collected after 8 weeks of same volume administration. The changes of blood pressure,trace albumin,and creatinine in rats were observed; local NADPH oxidase-4（ NOX4） levels,nuclear factor-κB（ NF-κB） p65,interleukin-6（ IL-6） and tumor necrosis factor-α（ TNF-α） protein expression levels of kidney were detected by Western blot; the effects of QYYYG on the release of these indicators were observed. Result： Compared with the normal group, the model gorup showed significant increase in blood pressure,trace albumin,and creatinine in rats,and NOX4,NF-κB p65,IL-6,and TNF-α protein expression levels in kidney of SHRs（ P 〈0. 01）. QYYYG can inhibit the expression of NOX4,reduce the NF-κB p65,IL-6,and TNF-α protein expression levels in kidney of SHRs,meanwhile it can reduce blood pressure,trace ablumin,and creatintine（ P 〈0. 01）,and the synergy effect was more obvious in case of combination of Qianyang Yuyin granules and Benazepril. Conclusion： QYYYG can improve the renal impairment of SHRs,and the mechanism may be associated with inhibiting oxidative stress and reducing the release of inflammation factors.

关 键 词：潜阳育阴颗粒 高血压肾损害 氧化应激 核转录因子-ΚB 自发性高血压大鼠 

分 类 号：R285.5[医药卫生—中药学]