桂枝茯苓胶囊及其活性成分组合物的抗大鼠盆腔炎作用  

Effect of Guizhi Fuling Capsule and Its Active Components on Pelvic Inflammation in Rats

作　　者：程玥[1] 唐朝辉[1] 曹亮[1] 丁岗[1] 王团结[1] 章晨峰[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国实验方剂学杂志》2016年第13期118-121,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203);现代中药创新集群与数字制药技术平台项目

摘　　要：目的:考察桂枝茯苓胶囊及其15个活性成分组合物对大鼠盆腔炎模型的影响,初步探讨其对盆腔炎的治疗作用。方法:90只雌性SD大鼠分为假手术组、模型组,模型组采用大肠埃希菌造成大鼠盆腔炎模型,将造模成功的大鼠随机分为模型组,桂枝茯苓胶囊组(250 mg·kg^(-1)),15个成分组合物低、中、高剂量组(75,150,300 mg·kg^(-1)),每组15只。桂枝茯苓胶囊及其成分组合物ig给药后15 d后将大鼠处死,取子宫后称重并计算子宫质量系数;取血后测血清肿瘤坏死因子-α(TNF-α),白细胞介素-2(IL-2)的含量及血浆免疫球蛋白A(Ig A),Ig G和Ig M的含量;苏木素-伊红(HE)染色检测大鼠子宫组织病理变化。结果:与假手术组比较,模型组大鼠子宫系数明显升高,血浆Ig A,Ig G和Ig M的含量明显降低,子宫病理综合评分明显升高(P<0.01);与模型组比较,桂枝茯苓胶囊及活性成分组合物高、中、低剂量组均能显著降低盆腔炎大鼠的子宫系数,能明显降低盆腔炎模型大鼠血清TNF-α含量,升高IL-2含量,并提高血浆Ig A,Ig G和Ig M的含量,对子宫病理综合评分也有显著性降低(P<0.05,P<0.01)。结论:15个活性成分组合物与桂枝茯苓胶囊均有明显的抗实验性大鼠盆腔炎作用,提示15个活性成分可能是桂枝茯苓胶囊盆腔炎的重要物质基础。Objective： To study the effect of Guizhi Fuling capsule and its 15 active components on pelvic inflammation in rat models, and preliminary investigate its treatment effect for pelvic inflammation.Method： Totally 90 female SD rats were divided into sham-operation group and model group. The pelvic inflammatory models were established by Escherichia coli. The successfully modeled rats were further randomly divided into model group,Guizhi Fuling capsule group（ 250 mg·kg^-1） and 15 active components low dose group,middle dose group and high dose group（ 75,150,300 mg·kg^-1）,n = 15 in each group. After intragastric administration for 15 d,the rats in Guizhi Fuling capsule group and active components groups were killed. Then their uterus was taken for weighing the uterus and calculating the uterus weight coefficient. Blood was obtained to measure tumour necrosis factor-α（ TNF-α）,interleukin-2（ IL-2） levels in serum and immune globulin A（ Ig A）,Ig G and Ig M levels in plasma. Pathological changes of uterine tissues were also detected by htoxylin eosin（ HE）staining method. Result： As compared with the sham-operation group, the uterus weight coefficient was significantly increased in rats of model group. Ig A,Ig G and Ig M levels in plasma were significantly reduced,and uterine pathology composite score was significantly increased（ P〈0. 01）. As compared with the model group,the uterus weight coefficient was significantly reduced in pelvic inflammatory rats of Guizhi Fuling capsule group and active components groups,TNF-α level in serum was significantly reduced; IL-2 level in serum was increased,the Ig A, Ig G and Ig M levels in plasma were increased; and uterine pathology composite score was also significantly reduced（ P〈 0. 05,P 〈0. 01）. Conclusion： The 15 active components and Guizhi Fuling capsule have significant effects on anti-pelvic inflammatory disease in rats,indicating that the 15 active components may be the main effective ingredients of Guizhi Fuling capsule in

关 键 词：桂枝茯苓胶囊 15个活性成分组合物 大鼠盆腔炎 肿瘤坏死因子-α 白细胞介素-2 免疫球蛋白 

分 类 号：R285.5[医药卫生—中药学]