藁本内酯抑制血管紧张素Ⅱ诱导的心肌肥大及其作用机制  

Ligustilide Inhibits AngⅡC-induced Cardiac Myocytes Hypertrophy

作　　者：黄美松 刘鹏[2] 王诗才 胡阳黔[2] 

机构地区：[1]十堰市中西医结合医院,湖北十堰442011 [2]湖北医药学院附属东风医院,湖北十堰442008

出　　处：《中国实验方剂学杂志》2016年第13期150-155,共6页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：研究藁本内酯（LIG）对血管紧张素Ⅱ（AngⅡ）诱导新生大鼠心肌细胞肥大的影响,并探讨其作用机制。方法：分离纯化SD大鼠心肌细胞后进行原代培养;将培养的心肌细胞经AngⅡ（0.5 mg·L）诱导和不同质量浓度LIG（20,40,80 mg·L^-1）作用1-3 d,另设空白组,在倒置显微镜下观察细胞形态,测定细胞表面积;运用BCA蛋白浓度测定方法测定各组心肌细胞中总蛋白含量;流式细胞术测定各组心肌细胞凋亡率;蛋白质免疫印迹（Western blot）测定各组心肌细胞中p53,Bcl-2和Bax蛋白表达情况。结果：与空白组比较,原代培养的新生大鼠心肌细胞经AngⅡ诱导后细胞形态发生明显变化,心肌细胞出现肥大效应,细胞表面积和细胞总蛋白含量明显增加（P〈0.05）,细胞凋亡率明显上升（P〈0.05）,诱导凋亡p53和Bax蛋白表达明显上升而抑制凋亡蛋白Bcl-2表达明显降低（P〈0.05）;与AngⅡ单独作用组比较,不同浓度LIG和AngⅡ联合作用能够明显抑制心肌细胞肥大效应,细胞表面积、细胞总蛋白含量及细胞凋亡均逐渐下降并呈剂量依赖效应（P〈0.05）。结论：LIG能够抑制由AngⅡ诱导的心肌细胞肥大,并可能通过诱导Bcl-2蛋白表达,抑制p53,Bax蛋白表达而降低细胞凋亡率发挥作用。Objective： To investigate the effect of ligustilide（ LIG） on angiotensin Ⅱ（ Ang Ⅱ）-induced cardiac myocytes hypertrophy and it＇s mechanism in neonatal rats. Method： Myocardial cells of SD rats were isolated and purified for primary culture. The cultured myocardial cells were induced by Ang Ⅱ（ 0. 5 mg·L^- 1） and treated with LIG（ various concentrations： 20,40,80 mg·L^- 1） for 1 to 3 days. Another blank group was established. Then the cells morphology was observed under inverted microscope and the cells surface area was determined. Total intracellular protein levels were detected by using commercial BCA protein kit and the rate of apoptosis was determined by flow cytometer. The protein expression levels of p53,Bcl-2 and Bax in myocardial cells were determined by Western blot. Result： As compared with the blank group,the cell morphology was obviously changed after exposure to Ang Ⅱ; hypertrophy effect was present in myocardial cells,the cells surface area,total protein levels,and apoptosis rate were increased significantly（ P 〈0. 05）. The protein expression levels of p53 and Bax were increased significantly,and the protein expression level of Bcl-2 was decreased significantly（ P 〈0. 05）. As compared with AngⅡ alone group,the combination effect of LIG with different concentrations and AngⅡ can ameliorate the AngⅡ-induced hypertrophy of neonatal rat myocardial cells,the cells surface area,total protein levels and apoptosis rate were decreased in a dose-dependent manner（ P 〈0. 05）. Conclusion： LIG can inhibit the AngⅡ-induced hypertrophy of cardiomyocytes,and reduce the apoptosis rate maybe by inducing Bcl-2protein expression and inhibiting p53 and Bax protein expressions.

关 键 词：心血管疾病 藁本内酯 血管紧张素Ⅱ 心肌肥大 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]