HPLC法测定小儿退热口服液中绿原酸和丹皮酚含量  

Determination of Chlorogenic Acid and Paeonol in Xiao'er Tuire Liquid by HPLC

作　　者：柯鹏颉 

机构地区：[1]福州市食品药品检验所,福州350000

出　　处：《海峡药学》2016年第5期61-63,共3页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC方法测定小儿退热口服液中绿原酸和丹皮酚含量。方法采用Welch Ultimate XB-C18（250mm×4.6mm,5μm）;以甲醇-0.2%磷酸为流动相,梯度洗脱;流速：1.0m L·min-1;检测波长：绿原酸：330nm,丹皮酚：278nm。结果绿原酸在0.50-25.06μg·m L-1范围内线性关系良好（r=0.9999）,平均加样回收率为100.60%,RSD为1.05%（n=6）;丹皮酚在1.23-61.52μg·m L-1范围内线性关系良好（r=1）,平均加样回收率为100.75%,RSD为0.55%（n=6）。结论该方法简单、准确、灵敏,重现性好,适用于小儿退热口服液的质量控制。OBJECTIVE To develop HPLC methods for determination of chlorogenic acid and paeonol in Xiao＇er Tuire Liquid. METHODS Geniposide was separated on Welch Ultimate XB-C18（ 250 mm × 4. 6mm,5μm） and flow rate was 1. 0m L · min- 1,methanol- 0. 2% phosphate was used as mobile phase. The detect wavelength for Chlorogenic acid was 330 nm and for Paeonol was 278 nm. RESULTS The linear range of chlorogenic acid was 50 -25. 06μg·m L- 1,r = 0. 9999. The average recovery was 100. 60% with a RSD of 1. 05%. The linear range of paeonol was 1. 23 - 61. 52μg · m L- 1,r = 1. The average recovery was 100. 75% with a RSD of 0. 55%. CONCLUSION The method is simple,sensitive,accurate with good reproducibility,and can be applied to the quality of Xiao＇er Tuire Liquid.

关 键 词：HPLC 小儿退热口服液 绿原酸 丹皮酚 

分 类 号：R927[医药卫生—药学]