PTTG基因对非霍奇金淋巴瘤细胞增殖能力的影响  

Effects of PTTG gene expression on the reproductive ability of non-hodgkin lymphoma cells

作　　者：徐理华[1] 刘瑞磊[2] 

机构地区：[1]广州医科大学附属第一医院血液科,广州510230 [2]中山大学附属第三医院甲乳外科

出　　处：《滨州医学院学报》2016年第3期173-175,179,共4页Journal of Binzhou Medical University

摘　　要：目的观察垂体瘤转化基因(pituitary tumor-transforming gene,PTTG)对非霍奇金淋巴瘤细胞SUDHL-4增殖能力的影响。方法通过shRNA的方法在高表达PTTG的SUDHL-4细胞中敲降PTTG基因,通过MTT实验、细胞周期分析检测SUDHL-4生物学行为的变化。结果 PTTG被敲降后SUDHL-4细胞的生长速度明显下降,细胞G0/G1比例、S期比例下降;G2/M期比例升高,细胞阻滞于G2/M期,差异均有统计学意义(P<0.05)。结论 PTTG基因可能在非霍奇金淋巴瘤的发生发展中起重要作用,可能成为判断非霍奇金淋巴瘤预后的分子标志物及临床治疗的靶点。Objective To explore the effects of PTTG gene expression on the reproductive ability of non-hodgkin lymphoma cells SUDHL-4.Methods Non-Hodgkin Lymphoma cells SUDHL-4was chose to show the biology behavior changes after silence PTTG by shRNA,through the MTT test,and cell cycle analysis.Results After silence PTTG by shRNA,the proliferation of SUDHL-4cells were significantly decreased,the ratio of G0/G1 and S phase cells was decreased,the ratio of G2/M cells was increased,and cell cycle arrested at G2/M,the difference was statistically significant（P〈0.05）.Conclusion The overexpression of PTTG gene may be related to the occurrence and development of lymphoma,and it may become the molecular diagnostics and new therapy target of lymphoma.

关 键 词：非霍奇金淋巴瘤 垂体瘤转化基因 SUDHL-4细胞 

分 类 号：R551.2[医药卫生—血液循环系统疾病]