海参寡肽海参寡肽:免疫调节作用及机制研究  

Sea cucumber oligopeptides:Immunomodulatory effects and its mechanism

作　　者：何丽霞[1] 陈启贺 刘睿[1] 任金威[1] 孙彬[1] 李林[1] 徐腾[1] 王军波[1] 张召锋[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院,北京100191

出　　处：《科技导报》2016年第11期42-47,共6页Science & Technology Review

基　　金：国家科技支撑计划项目(2006BAD27B08)

摘　　要：为研究海参寡肽对小鼠的免疫调节作用及机制,试验选取250只SPF级雌性BALB/c小鼠,随机分为5组:空白对照组,乳清蛋白组(0.30 g/kg),0.15、0.30、0.60 g/kg海参寡肽组。连续灌胃30 d后,通过测定Con A诱导的小鼠淋巴细胞转化实验、迟发型变态反应、抗体生成细胞、血清溶血素水平、小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验、自然杀伤(NK)细胞活性,观察海参寡肽对小鼠细胞免疫、体液免疫、单核-巨噬细胞吞噬和NK细胞活性的影响,并通过流式细胞术对脾脏T淋巴细胞亚群进行分析。结果表明:海参寡肽显著提高了小鼠细胞免疫、体液免疫、单核-巨噬细胞吞噬功能及NK细胞活性(P<0.05),且效果优于乳清蛋白。通过T淋巴细胞亚群分析表明,海参寡肽显著提高了脾脏CD3+百分比和CD4+百分比(P<0.05)。由此可知,海参寡肽可能通过增加T淋巴细胞数和Th细胞比例,介导细胞免疫、体液免疫功能、单核-巨噬细胞吞噬能力和NK细胞活性的增强作用,起到增强免疫功能的效果。To investigate the immunomodulatory effects of sea cucumber oligopeptides and their mechanisms, a total of 250 specific pathogen free （SPF） female BALB/c mice are randomly divided into 5 groups： the control group, the whey protein group （with 0.30 g/kg of whey protein） and the groups with 0.15, 0.30 and 0.60 g/kg of sea cucumber oligopeptides. The experiment takes 30 days. To determine the cell-mediated immunity, the humoral immunity, the macrophage phagocytosis and the natural killer （NK） cell activity in mice, the splenic lymphocyte proliferation and the delayed-type hypersensitivity assays （for the cell-mediated immunity）, the IgM antibody response of spleen to sheep red blood cells （SRBC） and the serum hemolysin level assays （for the humoral immunity）, the carbon clearance assay and the phagocytic capacity of peritoneal cavity phagocytes assays （for the macrophage phagocytosis） and the NK cell activity assays are performed. Furthermore, the flow cytometry is used to determine the T lymphocyte subsets. The results showed that the sea cucumber oligopeptides significantly increase the cell-mediated immunity, the humoral immunity, the macrophage phagocytosis and the NK cell activity in mice（P〈0.05）, and the effects are better than those of the whey protein. From the flow cytometry, it is observed that the sea cucumber oligopeptides significantly enhance the percentages of CD3^＋ and CD4^＋（P〈0.05）. In conclusion, the sea cucumber oligopeptides could increase the T lymphocyte cell number and the Th cell proportion to enhance the functions of the cell-mediated immunity, the humoral immunity, the macroohage ohagocytosis and the NK cell activity, and furthermore to improve the immune functions in mice.

关 键 词：海参寡肽 免疫功能 细胞免疫 体液免疫 巨噬细胞吞噬能力 自然杀伤细胞活性 T淋巴细胞 

分 类 号：R285.5[医药卫生—中药学]