基于NF-κB信号通路探讨小青龙汤合玉屏风散对变应性鼻炎大鼠MUC5AC和MUC5B表达的影响  

Exploring the Effect of Xiaoqinglong Decoction and Yupingfeng Powder on the Expression of MUC5AC and MUC5B in Rats with Allergic Rhinitis based on NF- kappa B Signaling Pathway

作　　者：王金昌[1] 马岩[1] 王树鹏[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2016年第6期16-18,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省教育厅课题(L2013374)

摘　　要：目的:基于NF-κB信号通路观察小青龙汤合玉屏风散对变应性鼻炎大鼠鼻黏膜MUC5AC和MUC5B表达的影响,揭示小青龙汤合玉屏风散治疗变应性鼻炎的作用机制。方法:将清洁级健康雄性Wistar大鼠48只按体重采用随机区组法分为6组,即分成正常组、AR模型组、Forskolin(c AMP激活剂)干预组、H89(PKA抑制剂)干预组、PDTC(NF-κB抑制剂)干预组、小青龙汤组合玉屏风散组,每组8只。采用卵蛋白全身致敏与局部攻击方法制作AR大鼠模型,观察小青龙汤合玉屏风散组治疗后鼻黏膜MUC5AC和MUC5B表达,结果进行统计学分析。结果:模型组MUC5AC和MUC5B表达上调,c AMP激活剂Forskolin干预组、NF-κB抑制剂PDTC干预组、小青龙汤组合玉屏风散治疗组均能使MUC5AC和MUC5B表达下调,而PKA抑制剂H89能上调MUC5AC和MUC5B表达。结论:小青龙汤合玉屏风散治疗变应性鼻炎作用机制可能与抑制NF-κB信号通路和兴奋c AMPPKA信号通路使MUC5AC和MUC5B表达下调有关。Objective：To observe the effect of the Xiaoqinglong Decoction and Yupingfeng Power on the expression of MUC5 B and MUC5 AC in nasal mucosa of allergic rhinitis rats by NF- kappa B signaling pathway,and reveal the mechanism of the Xiaoqinglong Decoction and Yupingfeng Power treating AR. Methods：48 healthy male wistar rats were divided into 6 groups by randomized bocks design：normal group,AR model group,Forskolin（c AMP activator）group,H89（PKA inhibitor）group,PDTC（NF-kappa B inhibitor）,and the Xiaoqinglong Decoction and Yupingfeng Power group,8 rats in each group. The AR rat model was made by the method of whole body sensitization and local attack. The expression of MUC5 AC and MUC5 B was observed after the treatment of the Xiaoqinglong Decoction and Yupingfeng Power group. The results were statistically analyzed. Results：The expression of MUC5 AC and MUC5 B in the model group increased,and the expression of MUC5 AC and MUC5 B in the Forskolin group,PDTC group,the Xiaoqinglong Decoction and Yupingfeng Power group decreased,while the PKA inhibitor H89 could increase the expression of MUC5 AC and MUC5 B. Conclusion：The mechanism of the treatment of allergic rhinitis by the Xiaoqinglong Decoction and Yupingfeng Power group may inhibit the expression of MUC5 AC and MUC5 B by inhibition of NF-kappa B signaling pathway and excitatory c AMP-PKA signaling pathway.

关 键 词：变应性鼻炎 小青龙汤 玉屏风散 MUC5AC MUC5B 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]