疮灵液调控慢性创面金属蛋白酶表达促进愈合的实验研究  

Improving Chronic Wound Healing With Traditional Chinese Medicine of Chuanglingye by the Way of Regulating the Expressions of MMPs in Wound

作　　者：许岩磊[1] 陈绪[1] 卞卫和[1] 姚昶[1] 朱永康[1] 朱小纾[2] 陈佳静[1] 叶小舟[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210046 [2]西悉尼大学生物和健康学院

出　　处：《辽宁中医药大学学报》2016年第6期25-28,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江苏省自然科学基金项目(BK2010598);江苏省中医药领军人才项目(LJ2009002);江苏省高校优势学科建设项目II期(012062003010);江苏省中医院高峰学术人才培养项目(y2014cr06)

摘　　要：目的：探查疮灵液对慢性创面肉芽金属蛋白酶表达的影响及促进创面愈合的部分作用机制。方法：采用大鼠背部创面金黄色葡萄球菌液注射模型,实验分为疮灵液组与对照组,疮灵液组以疮灵液覆盖创面,对照组以生理盐水纱布覆盖创面,隔日换药,术时、术后3 d、术后7 d、术后14 d创面照相测面积,取分泌物测TNF-α、IL-6含量及粒细胞总数,取肉芽测金属蛋白酶（MMP1、MMP2、MMP9）及其抑制剂（TIMP1）与羟脯氨酸（Hydroxyproline,Hyp）含量。结果：术后3～14 d,疮灵液组创面面积显著小于对照组,具有极显著统计学差异（P〈0.01）;疮灵液组创面分泌物中白细胞总数均显著少于对照组,14 d时,疮灵液组白细胞总数接近正常;疮灵液组创面分泌物中TNF-α、IL-6含量于术后7 d恢复正常,均显著低于对照组;术后3 d起,创面肉芽内MMP1、MMP2及MMP9均显著低于对照组（P〈0.01）,其中以MMP9表达影响疗效最显著,疮灵液对TIMP1未见明显影响;创灵液组在术后7、14 d肉芽中羟脯氨酸含量升高较明显,与对照组相比,有显著性差异。结论：疮灵液能够下调肉芽中金属蛋白酶含量,抑制慢性创面分泌物炎症因子分泌,并能够调节创面羟脯氨酸含量,从而促进慢性创面愈合。Objective：To explore the mechanisms of Chuanglingye on improving chronic wound healing through its functions of adjusting the expression of matrix metalloproteinases（MMPs）. Methods：The animal model of chronic wound was made by intramuscular injection of staphylococcus aureus on the back of SD rats. There were two groups in the experiment,which were treatment group（Chuanglingye group）with external application of Chuanglingye gauze and control group with saline gauze. The dressings were changed on each other day. On the day of model making and 3,7 and 14 days thereafter,the wound area,the content of TNF-α and IL-6 and the total number of neutrophile granulocyte in the wound exudate as well as the content of MMP1,MMP2 and MMP9,its inhibitor（TIMP1）and the hydroxyproline（Hydroxyproline）in the granulation tissue were tested respectively. Resluts：The wound areas in Chuanglingye group was statistically smaller than those in the control group from the 3rd to 14 th days after model making（P〈0.01）. Meanwhile,the total number of neutrophile granulocyte in Chuanglingye group was greatly less than those in control group. The number of neutrophile granulocyte in Chuanglingye group decreased to nearly normal value on day 14. The content of TNF-α and IL-6 in the Chuanglingye group recovered to normal value on the 7th day after model making,which was considerably less than those of control group. The contents of MMP1,MMP2 and MMP9 in the Chuanglingye group were remarkably less than those of control group since day 3（P〈0.01）,with the most differences happened on the expression of MMP9. However,the expression of TIMP1 did not change substantially in Chuanglingye group. The contents of Hyp in Chuanglingye group increased statistically on the 7th and 14 th day after model making as compared with the results of control group. Conclusion：Chuanglingye can not only inhibit the secretion of inflammatory factors by downregulating the expression of MMPs,but improve the level of hydroxyproline and thus th

关 键 词：疮灵液 慢性创面 炎症因子 金属蛋白酶 胶原 

分 类 号：R246.2[医药卫生—针灸推拿学]