东方田鼠自发性腹水细胞的分离培养与生物特性观察  

Culture and characterization of spontaneous ascites cells isolated from Microtus fortis

作　　者：成钢[1] 吴侠 尹检英 李淑红[1] 王京仁[1] 曾文虎[1] 

机构地区：[1]湖南文理学院动物学湖南省高校重点实验室,环洞庭湖生物资源保育与利用研究中心,常德415000 [2]广东省广州军区空军司令部门诊部

出　　处：《中国血吸虫病防治杂志》2016年第3期281-283,309,共4页Chinese Journal of Schistosomiasis Control

基　　金："动物学"湖南省"十三五"重点建设学科(湘教发201176);湖南文理学院大学生创新创业研究项目(YB1514)

摘　　要：目的对人工饲养条件下的东方田鼠自发性腹水细胞进行体外分离、培养及生物学特性进行初步观察,为研究东方田鼠腹腔微环境的抗虫机制提供实验依据。方法选取野外捕捉并人工饲养90 d后自发腹水的东方田鼠,分离培养腹水细胞,显微镜下观察细胞形态特征,并将其与正常及感染日本血吸虫的东方田鼠腹腔细胞进行对比;同时对组织病变进行观察。结果与正常及感染日本血吸虫后的东方田鼠腹腔细胞相比,自发性腹水东方田鼠无明显脏器组织病变,腹腔液中细胞数量和种类较多,细胞形态不规则,黏性大,贴壁后有大量空泡样细胞集落出现。随培养时间的延长,空泡样细胞集落依次呈现增殖、变形、崩解、塌陷及纤维样变化,纤维样腹水细胞传代3～4 d后死亡。结论东方田鼠自发性腹水细胞与其感染日本血吸虫后的腹腔细胞生物特性相似。Objective To isolate and culture the spontaneous ascites cells from Microtus fortis under artificial conditions,so as to investigate the molecular mechanism at the cell level. Methods The cells were isolated from spontaneous ascites of M.fortis artificially bred for 90 d,and were cultured and observed under a microscope. The differences of ascites cells among normal,spontaneous ascites and schistosomiasis infected samples of M. fortis were compared. The lesion of tissue was observed simultaneously. Results There were no obvious organ tissue lesions in M. fortis with spontaneous ascites,and the number and types of cells in peritoneal fluid were irregular and significantly changed. With the extension of culture time,the colonies appeared and there were a large number of vacuole-like cells in the cultured medium and sequentially presenting proliferation,deformation,disintegration and the fiber-like changes and could be passaged 3-4 d only. Conclusion The cells from M. fortis with spontaneous ascites are similar to its abdominal cavity cells after infection of Schistosoma japonica.

关 键 词：东方田鼠 腹水细胞 分离培养 生物学特性 

分 类 号：R532.21[医药卫生—内科学]