不同注射剂量的雷帕霉素对小型猪全身情况的影响  

Effect of different injection doses of rapamycin on general function changes in miniature pig

作　　者：朱钊[1] 庞宝兴[1] 吴晓珊[1] 胡磊[1] 胡亮[1] 王劲松[1] 张春梅[1] 王松灵[1] 

机构地区：[1]首都医科大学口腔医学院全牙再生与口腔组织功能重建北京市重点实验室,北京100050

出　　处：《首都医科大学学报》2016年第3期260-263,共4页Journal of Capital Medical University

基　　金：国家重点基础研究规划项目(973 Program)(2010CB944801);北京学者项目(PXM2014;PXM2013;TJSHG201310025005)~~

摘　　要：目的研究不同注射剂量的雷帕霉素对小型猪全身情况的影响。方法选择12头小型猪分为4组,连续7 d分别注射不同浓度的雷帕霉素(0、0.05、0.10、0.15 mg/kg),观察12周,期间每2周进行血常规和血生化检测。12周后,对小型猪主要脏器进行组织学观察。结果注射不同剂量的雷帕霉素2周后,各组小型猪的白细胞、血小板及淋巴细胞比率会出现剂量依赖性的下降,4周后恢复正常水平,血生化各项指标未见明显异常,12周后,小型猪的主要脏器的组织结构未见明显异常。结论连续7 d注射雷帕霉素不会对小型猪全身情况产生长期影响。Objective To evaluate the effect of different injection doses of rapamycin on general function changes in miniature pig（ minipig）. Methods Twelve minipigs were divided into 3 groups（ n = 3 per group）,which were injected with 0,0. 05,0. 10,0. 15 mg·kg- 1·day- 1for 7 continuous days separately. Blood samples were obtained every 2 weeks. The assessment of main viscera of minipigs12 weeks after injection was performed by hematoxylin and eosin（ HE） staining. Results The numbers of white blood cells,platelets and lymphocyte ratios were dose-dependently decreased at week 2 after injection,and returned to normal range at week 4. However,there was no significant difference in the main viscera of minipigs. Conclusion Seven continuous days injection with rapamycin had no impact on general condition of minipigs.

关 键 词：雷帕霉素 剂量依赖性 小型猪 

分 类 号：R979.5[医药卫生—药品]