茉莉酸信号转导途径介导番茄对棉铃虫的抗性  

Jasmonate signaling pathway mediates tomato resistance against Helicoverpa armigera

作　　者：崔伟康 杨鹏[1] 廉法卓 夏明[2] 徐金[1] 尤华才 陈冬梅[1] 宋圆圆[1,2] 

机构地区：[1]福建农林大学作物科学学院 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2016年第4期371-375,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31470477;31100286);福建省自然科学基金项目(2015J01091)

摘　　要：以过表达前系统素转基因番茄(35S∷prosys)、茉莉酸合成突变体番茄(spr2)和野生型番茄(WT)为试验材料,研究了其被棉铃虫取食后叶片保护酶活性和防御反应基因表达情况,以及对棉铃虫抗性的影响.结果表明:棉铃虫取食不同基因型番茄叶片后,多酚氧化酶、过氧化物酶、超氧化物歧化酶和脂氧合酶活性在35S∷prosys植株中最高,在WT植株中次之,在spr2植株中最低.荧光定量PCR检测表明,棉铃虫取食不同基因型番茄叶片后,脂氧合酶基因、丙二烯氧化物环化酶基因和蛋白酶抑制剂基因的转录水平在35S∷prosys植株中最高,在WT植株中次之,而在spr2植株中这些抗性基因的表达未受到虫害的诱导.生物测定表明,取食spr2的棉铃虫体重是取食WT和35S∷prosys棉铃虫体重的2.17和2.96倍.可见,茉莉酸信号途径介导了番茄对棉铃虫的抗性.Jasmonic acid （JA）-overexpression transgenic plants （35S：：prosys）, JA biosynthetic mutant （spr2） as well as corre-sponding wild type （ WT） of tomato （ Lycopersicon esculentum Miller） were planted, which was followed by being fed to Helicoverpa armigera. Results showed that 35S：：prosys plants demonstrated the highest induction of putative defensive enzymes, which include polyphenol oxidase, peroxidase, superoxide dismutase and lipoxygenase than the other gene types, and transcription of defense-relat-ed genes of lipoxygenase D, allene oxide cyclase and protease inhibitorsⅡin 35S：：prosys plants, which was higher than those of the WT plants, but no induction JA biosynthetic defective mutant spr2 plants. Bioassay showed that 35S：：prosys plants were the most re-sistant and spr2 plants were more susceptible to the caterpillars. These results suggest that JA signaling pathway mediates tomato re-sistance against H.armigera.

关 键 词：番茄 棉铃虫 茉莉酸信号途径 诱导抗虫性 

分 类 号：Q948.1[生物学—植物学]