番茄黄化曲叶病毒辽宁葫芦岛分离物的检测和序列分析  

Detection and sequence analysis on isolates of Tomato yellow leaf curl virus in Huludao,Liaoning Province

作　　者：杨彩霞[1] 张帅宗 孙蓬蓬[1] 高雅[1] 汪绪花 王喆[1] 

机构地区：[1]沈阳大学生命科学与工程学院/辽宁省城市有害生物治理与生态安全重点实验室,辽宁沈阳110044

出　　处：《福建农林大学学报（自然科学版）》2016年第4期376-380,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：辽宁省教育厅一般项目(L2015362);辽宁省自然科学基金(2015020806);辽宁省博士科研启动基金(20121043);沈阳大学博士科研启动基金(2012365)

摘　　要：采用已报道的菜豆金色花叶病毒属的通用引物PA/PB,从辽宁葫芦岛地区的3份番茄样品中扩增到Begomoviruses的保守区域,获得3个长度约500 bp的克隆序列,同源性为99.58%.DNA-A全基因组序列分析结果显示,3份样品DNA-A全长均为2 781 bp(分别命名为LNhud1、LNhud2和LNhud3分离物),具有典型双生病毒结构,与番茄黄化曲叶病毒(TYLCV)山东分离物(TYLCV-[CN:SD:SDWF-L7:12],KC999850)的核苷酸同源性最高(99.6%).根据双生病毒分类标准,认为LNhud1、LNhud2和LNhud3是TYLCV的辽宁分离物.系统关系树表明,这3个分离物属于TYLCV-Isreal株系.Universal degenerate primers PA/PB were used to detect Begomoviruses from 3 types of tomato plants showing leaf curl-ing symptoms in Huludao City, Liaoning Province, China. An approximately 500 bp fragments were amplified from each source of samples, and 99.58% sequence identity among partial DNA-A fragments confirmed that all three samples were infected by the same virus. Subsequent complete genome sequence analysis showed that DNA-A full length of all three isolates was 2 781 bp and were in the same genomic structure with begomoviral DNA-A. Three isolates were tentatively named LNhud1, LNhud2 and LNhud3, respec-tively. Basing on 99.6% nucleotide sequence identity, three isolates were most closely related to isolate of Tomato yellow leaf curl vi-rus （ TYLCV-[ CN：SD：SDWF-L7：12] , KC999850） from Shandong Province of China. Referring to demarcation criteria for identif-ying begomovirus species, LNhud1, LNhud2 and LNhud3 were considered as isolates of TYLCV and belonged to TYLCV-Isreal strain according to phylogenic analysis.

关 键 词：菜豆金色花叶病毒属 番茄黄化曲叶病毒 辽宁 序列分析 

分 类 号：S432.1[农业科学—植物病理学]