自组装近红外/磁共振双模态造影剂用于大鼠骨干细胞示踪  

Self-assembled NIRF/MRI Dual-modality Contrast Agents for Rat Bone Stem Cell( RBMSC) Tracking

作　　者：刘鸿[1,2] 谭燕[2] 韩雅莉[1] 王志勇[2] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]中国科学院深圳先进技术研究院,广东深圳518055

出　　处：《广东工业大学学报》2016年第4期62-68,共7页Journal of Guangdong University of Technology

基　　金：深圳市科技计划项目(JCYJ20140417113430662)

摘　　要：制备了适用于磁共振成像(MRI)和近红外成像(NIRF)的双模态造影剂,用于大鼠骨间充质干细胞的标记和示踪.以乙酰丙酮铁(III)、1,2-十六烷二醇、油酸和9-十八烯胺等为原料,通过热分解法制备单分散、粒度均匀的超顺磁氧化铁(SPIO)纳米粒子.再以硬脂酸修饰的聚乙烯亚胺(st PEI)为表面活性剂,通过自组装制备了同时携带SPIO和近红外染料IR-780的MRI和NIRF的双模态造影剂st PEI-SPIO/IR-780.经透射电镜、动态光散射、MRI和小动物活体成像仪系统表征显示该造影剂具有较合适的粒径、表面电势和出色的成像效果,用其标记、示踪大鼠骨髓间充质干细胞也表现出了很好的生物相容性和成像效果.In order to combine the advantages of magnetic resonance imaging （ MRI ） with near-infrared fluorescence （ NIRF ） imaging, a dual-modality contrast agent is prepared based on MRI and NIRF, which could be used for labeling and tracking rat bone mesenchymal stem cells （ RBMSCs） . Superpara-magnetic iron oxide （SPIO） nanocrystals are prepared by Fe（acac）3, 1,2-hexadecanediol, oleic acid, and oleylamine via thermal decomposition method, which posses monodisperse property and uniformly size. Then the contrast agents are used for magnetic resonance imaging （ MRI） and near-infrared fluores-cence （ NIRF） imaging by wrapping the SPIO nanocrystals and IR-780 dye with stearic acid-modified polyethylenimine （ stPEI ） self-assembly. Transmission electron microscopy （ TEM ） and dynamic light scattering （ DLS） are used to characterize the size and zeta potential, and MRI and IVIS Spectrum System used to characterize the imaging capability. The result shows that the contrast agents are reasonably nano-sized and positively charged. Moreover, the dual-modality contrast agent has showed effective bio and im-aging compatibility for labeling and tracking rat bone mesenchymal stem cells （ RBMSCs ） both in vitro and in vivo.

关 键 词：纳米粒子 磁共振成像 近红外成像 双模态造影剂 干细胞 

分 类 号：R445.2[医药卫生—影像医学与核医学]