高效液相色谱法对PHEMA液体栓塞材料中单体残留的超痕量分析  

Ultratrace Analysis of the Residual Monomer in Poly 2- Hydroxyethyl Methacry late Liquid Embolic Material by HPLC

作　　者：朱肖杰 朱小敏 马丽霞 王传栋 夏毅然 孟建文 

机构地区：[1]山东省药学科学院山东省医用高分子材料重点实验室,济南250101

出　　处：《生物医学工程研究》2016年第2期136-138,共3页Journal Of Biomedical Engineering Research

摘　　要：应用高效液相色谱法测定了聚甲基丙烯酸-β-羟乙酯（PHEMA）液体栓塞材料中的单体甲基丙烯酸-β-羟乙酯（HEMA）残留量。采用色谱柱Aichrom Bond-1 C18（4.6 mm×250 mm,5μm）进行分离,流动相为乙腈-水（30∶70）;检测波长为220 nm;进样量为20μL。单体在质量浓度为0.1~4.0 mg/L的范围,峰面积与单体浓度线性关系良好,相关系数0.9992,检测下限为0.01 mg/L。当试样加标浓度为0.5,2.0,4.0 mg/L时,加标回收率在99.5%~102.0%之间,RSD≤2%。A method for determination of the residual monomer in poly 2- hydroxyethyl methacrylate（ PHEMA） liquid embolic material by HPLC was established. Aichrom Bond- 1 C18 column（ 4. 6mm × 250 mm,5μm） was used as stationary phase and acetonitrile- H2 O mixture（ 30： 70） was used as mobile phase in the separation. Ultraviolet detector at 220 nm was adopted in the determination.The monomer showed a good calibration linearity with the range of 0. 1 ~ 4. 0 mg / L and the correlation coefficient was 0. 9992. The detection limit is 0. 01 mg / L. When the sample concentration is 0. 5,2. 0,4. 0 mg / L,the recoveries are ranged from 99. 5% to 102. 0%,and RSD≤2%.

关 键 词：高效液相色谱法 液体栓塞材料 甲基丙烯酸-Β-羟乙酯 单体残留 检测 

分 类 号：R318[医药卫生—生物医学工程]