一种基于农杆菌介导的拟南芥瞬时转化技术优化  

Optimizing Transient Genetic Transformation Method on Arabidopsis Plants Mediated by Agrobacterium tumefaciens

作　　者：郭勇[1] 王玉成[1] 王智博[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《东北林业大学学报》2016年第6期41-44,83,共5页Journal of Northeast Forestry University

基　　金：哈尔滨市科技创新人才研究专项资金(优秀学科带头人)项目(2012RFXXN023)

摘　　要：利用根癌农杆菌(Agrobacterium tumefaciens)介导的拟南芥瞬时转化体系影响因素来确定最佳转化条件。以生长15日龄的拟南芥幼苗为试验材料,以转化p CAMBIA1301空载体的根癌农杆菌EHA105为目的菌株进行瞬时转化。研究了吐温20、菌液OD值、乙酰丁香酮(AS)及转化时间等对拟南芥瞬时转化效率的影响。结果表明:以体积分数为0.05%的吐温、菌液OD_(600)值为1.0和120μmol/L的AS侵染拟南芥2.5 h后,再共培养72 h,能够得到高瞬时转化效果。We studied the optimal transformation conditions for transient genetic transformation of Arabidopsis mediated by Agrobacterium tumefaciens. We transformed Agrobacterium EHA105 carrying an empty pC AMBIA1301 into the 15-day Arabidopsis plants for genetic transformation research. We studied the effects on transient expression including the concentration of the Agrobacterium,Tween-20,and acetosyringone（ AS）,and transformed time. Under the conditions of 1.0 OD of Agrobacterium,0.05% of Tween-20,120 μmol / L of AS,transformed for 2.5 hours,and co-cultured for 3 days,the transient transformation efficiency is highest.

关 键 词：拟南芥 GUS基因 瞬时表达 实时定量 农杆菌介导转化 

分 类 号：Q786[生物学—分子生物学]