银杏内酯B对高糖诱导人晶状体上皮细胞凋亡的影响  被引量:3

Effect of ginkgolide B on high glucose-induced human lens epithelial cell apoptosis

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作  者:王彦红[1] 兰天野[1] 陈婷[1] 刘平[1] 林丽丽[1] 

机构地区:[1]哈尔滨医科大学附属第一医院眼科,中国黑龙江省哈尔滨市150001

出  处:《国际眼科杂志》2016年第7期1242-1244,共3页International Eye Science

基  金:国家自然科学基金(No.30973275)~~

摘  要:目的:探讨银杏内酯B对高糖诱导的人晶状体上皮细胞(human lens epithelial cells,HLECs)凋亡的影响。方法:建立高糖诱导的HLECs模型,用不同浓度的银杏内酯B干预,MTT比色法检测细胞存活率,Hochest33258染色观察凋亡细胞形态,透射电镜观察细胞内超微结构变化,比色法检测凋亡相关因子Caspase-3及Caspase-9的表达。结果:高浓度葡萄糖可抑制HLECs活性,诱导HLECs发生凋亡,引起细胞内Caspase-3及Caspase-9表达水平升高;银杏内酯B能明显抑制高糖诱导的HLECs活力的下降,降低细胞凋亡率,减少高糖所致HLECs细胞内Caspase-3及Caspase-9的表达。结论:银杏内酯B可能通过抑制Caspase-3及Caspase-9的表达而有效抑制了高糖诱导的HLECs凋亡。AIM: To study the effect of ginkgolide B on high glucose-induced human lens epithelial cell (HLEC) apoptosis. METHODS: The high glucose-induced HLEC model was established. Different concentrations of ginkgolide B were intervened. Cell viability was assayed by MTT assay, morphology of cell apoptosis was observed by hochest33258 staining. Cell ultrastructural changes were detected by transmission electron microscopy. Expressions of apoptosis - related factors caspses - 3 and caspase-9 were tested by colorimetric detection. RESULTS: High glucose inhibited the activity of HLECs, induced apoptosis reaction of HLECs, caused high expression of apoptosis factors in cells; while ginkgolide B inhibited the decrease of cell viability induced by high glucose, decreased the HLEC apoptosis and reduced the expression of apoptosis factors Caspase-3 and Caspase-9.CONCLUSION: Ginkgolide B may inhibit the expression of caspses-3 and caspase-9 then effectively inhibited high glucose-induced apoptosis in HLECs.

关 键 词:银杏内酯B 高糖 晶状体上皮细胞 凋亡 

分 类 号:R587.2[医药卫生—内分泌] R776.1[医药卫生—内科学]

 

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