15个白三叶品种的ISSR和ITS遗传多样性分析  

ISSR and ITS analysis on genetic diversity of 15 white clover cultivars

作　　者：李金博 高丽华[2] 周美亮[2] 李诗刚 彭昭良 吴燕民[2] 

机构地区：[1]兰州大学草地农业科技学院,甘肃兰州730020 [2]中国农业科学院生物技术研究所,北京100081 [3]深圳市铁汉生态环境股份有限公司,广东深圳523000

出　　处：《草业科学》2016年第6期1147-1153,共7页Pratacultural Science

基　　金：国家自然科学基金面上项目(31572457);铁汉生态研究院建设(2014B090903015);铁汉生态院士工作站建设(2015B090904008)

摘　　要：利用ISSR标记和ITS序列分析在我国大面积推广应用的15个白三叶(Trifolium repens)品种的遗传多样性与地理来源关系,试图为我国白三叶新品种的选育提供遗传理论支持。结果表明,15个白三叶品种明显分为两大类,第Ⅰ类有10个白三叶品种,主要来源于丹麦、澳大利亚、新西兰和荷兰;第Ⅱ类有5个白三叶品种,主要来源于美国、中国和阿根廷。白三叶品种聚类具有一定的地域性分布规律,ISSR和ITS均能有效地对白三叶品种进行遗传多样性分析,且ISSR可以更加细致地对其进行分类。The relationship of genetic diversity with geographical origin between 15 white clover cultivars on the popularization and application of the large area in China,which provides genetic theory support the white clover breeding.The results showed that 15 white clover cultivars were divided into two categories,the classⅠ has10 cultivars of white clover,mainly comes from Denmark,Australia,New Zealand and the Netherlands;There are five white clover cultivars in the classⅡ,mainly comes from USA,China and Argentina.White clover cultivars clustering has certain regional distribution,the genetic diversity of white clover cultivars could be studied by using ISSR and ITS analyzing,and ISSR can be more detailed on the classification.

关 键 词：白三叶 ISSR ITS 遗传多样性 

分 类 号：S541.2[农业科学—作物学]