秋水仙素对花生的诱导研究  

Induction Effect of Colchicine on Peanut

作　　者：梅辉[1] 韦琴 付秀芹[1] 江楠[1] 

机构地区：[1]武汉生物工程学院生命科学与技术学院,湖北武汉430415 [2]武汉工商学院环境与生物工程学院,湖北武汉430065

出　　处：《安徽农学通报》2016年第12期36-37,共2页Anhui Agricultural Science Bulletin

基　　金：武汉市教育局科研项目(2009K089):秋水仙素对花生的诱导研究

摘　　要：研究用不同浓度的秋水仙素(0.002%、0.01%、0.02%、0.1%、0.2%)对花生种子进行不同时间(2h、4h、6h、8h、10h)的诱导处理,分析种子发芽率及生长速率受秋水仙素浓度和作用时间的影响,并对花生根尖细胞的倍性进行鉴定。结果表明:秋水仙素对花生种子的发芽率和生长速率有显著的抑制作用,抑制作用随秋水仙素浓度和作用时间的增加而递增;诱导花生多倍体的最佳作用浓度和作用时间为0.01%秋水仙素处理4h或者0.02%秋水仙素处理2h。With different concentrations of colchicine （0.002%, 0.01%, 0.02%, 0.1%, 0.2%）on peanut seeds of dif- ferent time （2h, 4h, 6h, 8h, 10h）treatment induced, analysed the effect of colchicine concentration and action time of germination rate and the growth rate of peanut seeds, and also the root tip cells were identified.The results showed that there was significant inhibitory effect of colchicine on germination ratio and growth rate of peanut, and the inhibition was increased by increasing the concentration and action time of colchicine.The optimal concentration and time of induction of polyploid peanut is 0.01%/4h or 0.02%/2h.

关 键 词：秋水仙素 花生 发芽率 生长速率 

分 类 号：S565[农业科学—作物学]